HPLC Analysis of Zearalenone and Ochratoxin A on Primesep B2 Mixed-Mode Column

Zearalenone and Ochratoxin A are harmful mycotoxins commonly found in grains and other foods. They were separated using the Primesep B2 reversed-phase anion-exchange mode column. Retention can be controlled by adjusting concentration of both acetonitrile and buffer.

Condition

Column Primesep B2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Base, Acid, Hydrophilic, Ionizable
Analyzing Compounds Zearalenone, Ochratoxin A

 

Application Column

Primesep B2

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ochratoxin A
Zearalenone

Application Detection:
ELSD Detection
UV Detection
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