Pefloxacin

Pefloxacin

CAS Number70458-92-3
Molecular FormulaC17H20FN3O3
Molecular Weight333.363
InChI KeyFHFYDNQZQSQIAI-UHFFFAOYSA-N
LogP0.27
Synonyms
  • Pefloxacin
  • 1-Ethyl-6-fluoro-7-(4-methylpiperazin-1-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylic acid
  • 3-Quinolinecarboxylic acid, 1-ethyl-6-fluoro-1,4-dihydro-7-(4-methyl-1-piperazinyl)-4-oxo-
  • 70458-92-3
  • 3-Quinolinecarboxylic acid, 1-ethyl-6-fluoro-1,4-dihydro-7-(4-methyl-1-piperazinyl)-4-oxo-
  • Abactal
  • Abaktal
  • Globacin
  • N'-Methylnorfloxacin
  • Peflacine
  • pefloxacine
  • pefloxacino
  • BRN 0567618
  • EINECS 274-611-8
  • 1-Ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(4-methyl-1-piperazinyl)-3-quinolinecarboxylic acid
  • Pefloxacinum
  • UNII-2H52Z9F2Q5
  • PFLX
  • perfloxacin

Applications:

HPLC Method for Analysis of Pefloxacin on Primesep 100 Column

August 9, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pefloxacin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Pefloxacin on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Pefloxacin

Pefloxacin is an antibiotic that belongs to the fluoroquinolone class of drugs. It is primarily used to treat bacterial infections by inhibiting the DNA gyrase enzyme, which is essential for bacterial DNA replication and repair. Because of its mechanism, Pefloxacin is effective against a variety of gram-negative and some gram-positive bacteria.

Pefloxacin can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.

You can find detailed UV spectra of Pefloxacin and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Limit of Detection40 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
quinolinemonocarboxylic acid
Analyzing CompoundsPefloxacin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Pefloxacin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Pefloxacin

June 28, 2024

Uv-Vis Absorption Spectrum of Pefloxacin

 

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

Application Analytes:
Pefloxacin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of a Complex of Quinolone Antibiotics

May 27, 2015

Quinolones are a group of synthetic broad-spectrum antibiotics with a wide variety of applications in medicine and veterinary sciences. Some quinolones have been found in food products including fish and shrimp. Primesep 100 was used to separate a mixture of quinolones and achieve baseline separation. Retention was controlled by adjusting acetonitrile in the mobile phase.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Quinolone Antibiotics, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Oxolinic Acid, Flumequine, Ciprofloxacin, Enrofloxacin, Pefloxacin

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ciprofloxacin
Enrofloxacin
Flumequine
Oxolinic acid
Pefloxacin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.