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Applications:
HPLC Analysis of Apple Extract using Mixed-Mode Chromatography
2010-05-06
Apple extract is a complex mixture of polyphenols, vitamins and amino acids. Analysis of this mixture is performed by mixed-mode chromatography. Different selectivity is achieved on two different columns: Primesep 200 reversed-phase cation-exchange column, and Primesep D reversed-phase anion-exchange HPLC column. Compounds are monitored by UV detection. Methods are compatible with LC/MS and preparative chromatography and can be used for identification and isolation of specific compounds.
Application Column
Primesep 200
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select options
Primesep D
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsApplication Analytes:
Apple ExtractApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
