| CAS Number | 18389-49-6 |
|---|---|
| Molecular Formula | C10H15N5O10P2 |
| Molecular Weight | 427.203 |
| InChI Key | XTWYTFMLZFPYCI-KQYNXXCUSA-N |
| LogP | -4.6 |
| Synonyms |
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Applications:
HPLC Separation of Adenosine mono-, di- and triphosphates on Newcrom BH Column
May 11, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine monophosphate (AMP), Adenosine diphosphate (ADP), Adenosine triphosphate (ATP)
Adenosine mono-, di- and triphosphates are key components of the cell’s energy ecosystem. Adenosine triphosphate (ATP) is the main unit of energy used in the cell. When it is ‘spent,’ it is converted to adenosine diphosphate (ADP); it is often converted back to ATP to be reused. Adenosine monophosphate can be converted to ADP (and subsequently ATP), but it is also a key factor in RNA synthesis.
Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile with a sulfuric acid (H2SO4) buffer, adenosine mono-, di- and triphosphate can be separated, measured, and analyzed. This method can UV detect this family of compounds at 262 nm with very high resolution.
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 262nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Adenosine monophosphate (AMP) Adenosine diphosphate (ADP) Adenosine triphosphate (ATP) |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsAdenosine Monophosphate
Adenosine Triphosphate
HPLC Separation of Adenosine Mono-, Di- and Triphosphate on Newcrom B column
April 13, 2020
| Column | Newcrom B, 4.6×150 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 1.0% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 262nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Adenosine monophosphate (AMP) Adenosine diphosphate (ADP) Adenosine triphosphate (ATP) |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsAdenosine Monophosphate
Adenosine Triphosphate
Separation of Nine Nucleotides by Mixed-Mode Chromatography
July 6, 2015
Nucleotides are important biological molecules which serve as subunits of nucleic acids. They are composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Nucleotides cannot be retained by reverse-phase chromatography without an ion-pairing reagent due to their highly polar nature. Primesep SB is capable of retaining and separating nine nucleotides. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups.
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAdenosine Monophosphate
Adenosine Triphosphate
Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate
Guanosine Diphosphate
Guanosine Monophosphate
Guanosine Triphosphate
HPLC Separation of Adenosine Mono-, Di- and Triphosphate in Reversed-Phase Mixed-Mode with LC/MS Compatible Conditions
December 5, 2013
Adenosine mono-, di and triphosphate are hydrophilic nucleotides which serve as building blocks of DNA and RNA. Each molecule consists of phosphate or phosphate groups, adenine and sugar ribose. Molecules are hydrophilic and lack a retention mechanism on traditional reversed-phase column. Three nucleotides were retained and separated on Primesep B2 reversed-phase anion-exchange column. retention time is controlled by buffer concentration and buffer pH. ADP and ATP require higher concentration of buffer to facilitate elution. Method can be used for LC/MS analysis of different nucleotides in various sample matrices (biofluids, plasma, blood, urine). Other detection techniques can be used for analysis. Method is reliable and robust and can tolerate interference from sample matrix. Additional sample preparation might be required.
| Column | Primesep B2, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 2.9- 5-20 mM 3 min, 20-150 mM |
| Flow Rate | 0.5 ml/min |
| Detection | ELSD |
| Class of Compounds |
Nucleotide, Hydrophilic, Ionizable |
| Analyzing Compounds | Adenosine Monophosphate, Adenosine Diphosphate, Adenosine Triphosphate |
Application Column
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAdenosine Monophosphate
Adenosine Triphosphate
