Adenosine Diphosphate

CAS Number18389-49-6
Molecular FormulaC10H15N5O10P2
Molecular Weight427.203
InChI KeyXTWYTFMLZFPYCI-KQYNXXCUSA-N
LogP-4.6
Synonyms
  • Adenosine 5'-diphosphate
  • Adenosine diphosphate
  • ADP
  • adenosine-5'-diphosphate
  • adenosine pyrophosphate
  • Adenosine 5'-(trihydrogen diphosphate)
  • adenosine 5'-pyrophosphate
  • ADP (nucleotide)
  • adenosine-diphosphate
  • 5'-Adenylphosphoric acid
  • 5'-Adp
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphoric acid
  • Adenosine-5'-diphosphat
  • Adenosine 5'-diphosphoric acid
  • Adenosine diphosphoric acid
  • Ado-5'-P-P
  • UNII-61D2G4IYVH
  • H3adp
  • BRN 0067722
  • CHEBI:16761
  • EINECS 200-392-5
  • CHEMBL14830
  • XTWYTFMLZFPYCI-KQYNXXCUSA-N
  • ADENOSINE, 5'-(TRIHYDROGEN PYROPHOSPHATE)
  • 20398-34-9
  • AK-54732
  • 84412-16-8
  • adenosine-5-diphosphate

Applications:

HPLC Separation of Adenosine mono-, di- and triphosphates on Newcrom BH Column

May 11, 2021

Separation type: Liquid Chromatography Mixed-mode









High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine monophosphate (AMP), Adenosine diphosphate (ADP), Adenosine triphosphate (ATP)




Adenosine mono-, di- and triphosphates are key components of the cell’s energy ecosystem. Adenosine triphosphate (ATP) is the main unit of energy used in the cell. When it is ‘spent,’ it is converted to adenosine diphosphate (ADP); it is often converted back to ATP to be reused. Adenosine monophosphate can be converted to ADP (and subsequently ATP), but it is also a key factor in RNA synthesis.
Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile with a sulfuric acid (H2SO4) buffer, adenosine mono-, di- and triphosphate can be separated, measured, and analyzed. This method can UV detect this family of compounds at 262 nm with very high resolution.




Condition

Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 262nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds Adenosine monophosphate (AMP)
Adenosine diphosphate (ADP)
Adenosine triphosphate (ATP)

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Adenosine Mono-, Di- and Triphosphate on Newcrom B column

April 13, 2020


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Condition

Column Newcrom B, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H2SO4 – 1.0%
Flow Rate 1.0 ml/min
Detection UV 262nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds Adenosine monophosphate (AMP)
Adenosine diphosphate (ADP)
Adenosine triphosphate (ATP)

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Nine Nucleotides by Mixed-Mode Chromatography

July 6, 2015

 

Nucleotides are important biological molecules which serve as subunits of nucleic acids. They are composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Nucleotides cannot be retained by reverse-phase chromatography without an ion-pairing reagent due to their highly polar nature. Primesep SB is capable of retaining and separating nine nucleotides. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups.

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate
Guanosine Diphosphate
Guanosine Monophosphate
Guanosine Triphosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Adenosine Mono-, Di- and Triphosphate in Reversed-Phase Mixed-Mode with LC/MS Compatible Conditions

December 5, 2013

Adenosine mono-, di and triphosphate are hydrophilic nucleotides which serve as building blocks of DNA and RNA. Each molecule consists of phosphate or phosphate groups, adenine and sugar ribose. Molecules are hydrophilic and lack a retention mechanism on traditional reversed-phase column. Three nucleotides were retained and separated on Primesep B2 reversed-phase anion-exchange column. retention time is controlled by buffer concentration and buffer pH. ADP and ATP require higher concentration of buffer to facilitate elution. Method can be used for LC/MS analysis of different nucleotides in various sample matrices (biofluids, plasma, blood, urine). Other detection techniques can be used for analysis. Method is reliable and robust and can tolerate interference from sample matrix. Additional sample preparation might be required.

 

Condition

Column Primesep B2, 3.2×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm pH 2.9- 5-20 mM 3 min, 20-150 mM
Flow Rate 0.5 ml/min
Detection ELSD

 

Description

Class of Compounds
Nucleotide, Hydrophilic, Ionizable
Analyzing Compounds Adenosine Monophosphate, Adenosine Diphosphate, Adenosine Triphosphate

 

Application Column

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.