HPLC Separation of Adenosine Mono-, Di- and Triphosphate on Newcrom B column

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Column Newcrom B, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H2SO4 – 1.0%
Flow Rate 1.0 ml/min
Detection UV 262nm


Class of Compounds Acid, Hydrophilic
Analyzing Compounds Adenosine monophosphate (AMP)
Adenosine diphosphate (ADP)
Adenosine triphosphate (ATP)


Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.