HPLC Separation of Adenosine mono-, di- and triphosphates on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode



High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine monophosphate (AMP), Adenosine diphosphate (ADP), Adenosine triphosphate (ATP)

Adenosine mono-, di- and triphosphates are key components of the cell’s energy ecosystem. Adenosine triphosphate (ATP) is the main unit of energy used in the cell. When it is ‘spent,’ it is converted to adenosine diphosphate (ADP); it is often converted back to ATP to be reused. Adenosine monophosphate can be converted to ADP (and subsequently ATP), but it is also a key factor in RNA synthesis. Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile with a sulfuric acid (H2SO4) buffer, adenosine mono-, di- and triphosphate can be separated, measured, and analyzed. This method can UV detect this family of compounds at 262 nm with very high resolution.

Condition

Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer H2SO4 - 0.2%
Flow Rate 1.0 ml/min
Detection UV 262nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds Adenosine monophosphate (AMP) Adenosine diphosphate (ADP) Adenosine triphosphate (ATP)
 
Application Analytes:
Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate