Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. Obelisc N HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved by hydrophilic interaction chromatography. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (ultra-violet, ELSD, LC/MS, RI, CAD, etc.)
| Column | Obelisc N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250, 230nm |
| Class of Compounds |
Surfactant, Hydrophobic, Ionizable |
| Analyzing Compounds | Triton X-100 |
Application Column
Obelisc N
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
Triton X100Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
