Salicylic acid

Salicylic acid structural formula

UV- Vis Absorption Spectrum of Salicylic Acid λ max 204, 238, 304

CAS Number69-72-7
Molecular FormulaC7H6O3
Molecular Weight138.122
InChI KeyYGSDEFSMJLZEOE-UHFFFAOYSA-N
LogP2.26
Synonyms
  • Salicylic acid
  • 2-Hydroxybenzoic acid
  • Benzoic acid, 2-hydroxy-
  • 69-72-7
  • Salicyclic acid (SA) (Benzoic acid, 2-hydroxy-)
  • Benzoic acid, 2-hydroxy-
  • 2-Carboxyphenol
  • 2-Hydroxybenzenecarboxylic acid
  • 2-Hydroxybenzoic acid
  • Acide salicylique
  • acido salicilico
  • Advanced Pain Relief Callus Removers
  • Advanced Pain Relief Corn Removers
  • Clear away Wart Remover
  • Compound W
  • Dr. Scholl's Callus Removers
  • Dr. Scholl's Corn Removers
  • Dr. Scholl's Wart Remover Kit
  • Duofil Wart Remover
  • Duoplant
  • Freezone
  • Ionil Plus
  • NSC 180
  • o-Carboxyphenol
  • o-Hydroxybenzoic acid
  • ORTHOHYDROXY BENZOIC ACID
  • Phenol-2-carboxylic acid
  • Psoriacid-S-Stift
  • Retarder W
  • Rutranex
  • SALICYCLIC ACID
  • Salicylic acid collodion
  • Salicylic Acid Soap
  • SALICYLSAEURE
  • Salicylsaure
  • Saligel
  • Salonil
  • Salvona Nanosal
  • Salycilic acid
  • Salysilic acid
  • Stri-Dex
  • Trans-Ver-Sal
  • BRN 0774890
  • Caswell No. 731
  • EINECS 200-712-3
  • EPA Pesticide Chemical Code 076602
  • Orthohydroxybenzoic acid
  • Salicylic acid, tech.
  • Verrugon
  • Acidum salicylicum
  • Salex Cream
  • Salex Lotion
  • P&S Shampoo
  • Propa pH Peel-Off Acne Mask
  • Akurza Cream
  • Akurza Lotion
  • DHS Sal Shampoo
  • DuoPlant Gel
  • Hydrisalic Gel
  • Mediplast pads
  • Acido o-idrossibenzoico
  • Kyselina 2-hydroxybenzoova
  • Kyselina salicylova
  • UNII-O414PZ4LPZ
  • Durasal
  • salicylicum acidum
  • 2-Hydroxybenzenecarboxylate
  • 2-Hydroxybenzoate
  • Clear away Wart Remover
  • Ionil
  • K 537
  • K 557
  • O-Hydroxybenzoate
  • Phenol-2-carboxylate
  • Salicylate
  • Trans-Ver-Sal
  • 1186130-36-8
  • 7681-06-3
  • 8052-31-1

Applications:

HPLC Method For Analysis Of Salicylic Acid on Newcrom BH Column

May 2, 2022

Separation type: Liquid Chromatography Mixed-mode


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Salicylic acid (SA) is an o-hydroxybenzoic acid, synthesized by both plants and microorganisms. SA acts as a critical plant hormone regulating various processes, including growth and development, flowering, thermogenesis, ion uptake, stomatal movement, photosynthesis, and plant immunity. SA also has medical applications as a topical treatment for a wide variety of skin conditions, including warts, dandruff, and acne. Using a Newcrom BH mixed-mode column and a mobile phase consisting of water, Acetonitrile (MeCN), and a Phosphoric acid (H3PO4) buffer, Salicylic acid can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Salicylyc Acid

Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase MeCN – 40%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds Salicylic Acid

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Salicylic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Cinnamic Acid, Salicylic Acid and Quercetin on Newcrom B Column

March 12, 2021

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method of Cinnamic Acid, Salicylic Acid and Quercetin.

Cinnamic acid and benzoic acid are two of the major phenolic acids and autotoxins. Cinnamic acid occurs naturally in all green plants, showing significant antimicrobial activity against both bacteria and fungi as well as antioxidant activity. Salicylic acid (SA) is an o-hydroxybenzoic acid, synthesized by both plants and microorganisms. SA acts as a critical plant hormone regulating various processes, including growth and development, flowering, thermogenesis, ion uptake, stomatal movement, photosynthesis, and plant immunity. Quercetin is a naturally occurring flavonol, or flavonoid, the yellowish antioxidant pigment found in skins of red grapes, berries, apples, onions, tomatoes, and buckwheat tea. In addition to functioning as a flavonoid, quercetin is also a phytoestrogen. All three compounds can be retained and separated by using a mixed-mode Newcrom B column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid (H3PO4) buffer. UV detection at 250 nm.



Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN -50%
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection 250 nm

Description

Class of Compounds Acid
Analyzing Compounds Cinnamic acid, Quercetin, Salicylic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Cinnamic acid
Quercetin
Quercetin dihydrate
Salicylic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Phenolic Acids

March 11, 2021


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Condition 1

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection 270 nm

Condition 2

Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection 270 nm

Description

Class of Compounds Acid
Analyzing Compounds Gallic acid, 3,4 Dihydroxybenzoic acid, Caffeic acid, trans-Ferulic acid, trans- p -Coumaric acid, trans-Cinnamic acid, Quercetin Hydrate, Salicylic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
3,4-Dihydroxybenzoic Acid
Caffeic acid
Cinnamic acid
Ferulic acid
Gallic acid
Quercetin
Quercetin dihydrate
Salicylic acid
p-coumaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid on Primesep B Column

August 14, 2019

HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid_1182

2-Hydroxyethyl salicylate is a drug used as analgesic agents for the treatment of mild to moderate pain.

 

Condition

Column Primesep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 25/75%
Buffer H2SO4 – 0.5 %
Flow Rate 1.0 ml/min
Detection UV, 238 nm

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Carbocyclic, Benzoate, Hydroxy Acid, Hydroxybenzoate, Phenol
Analyzing Compounds 2-Hydroxyethyl Salicylate, Salicylic Acid

 

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Hydroxyethyl salicylate
Salicylic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Salicylic acid

August 6, 2015

 

 

 

Salicylic acid is a pain-reliever and anti-inflammatory commonly found in rubefacient products for topical pain relief as well as cosmetic skin-care products used to treat a variety of skin conditions. Legacy L1 was used to retain salicylic acid by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

Condition

Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeOH – 45%
Buffer Acetic acid – 0.55%
Flow Rate 1.0 ml/min
Detection UV, 280 nm

 

 

Description

Class of Compounds
 Acid,  Hydrophilic, Ionizable
Analyzing Compounds Salicylic acid

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
Salicylic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.