| CAS Number | 1701-77-5 |
|---|---|
| Molecular Formula | C9H10O3 |
| Molecular Weight | 166.176 |
| InChI Key | DIWVBIXQCNRCFE-UHFFFAOYSA-N |
| LogP | 1.20 |
| Synonyms |
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Applications:
HPLC Chiral Separation of alpha-Methoxyphenylacetic acid on Cyralis CM Column
June 17, 2026
HPLC Method for Analysis of Methoxyphenylacetic acid on Cyralis CM Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Methoxyphenylacetic acid.
alpha-Methoxyphenylacetic acid is a chiral carboxylic acid with the chemical formula C9H10O3. It is primarily used as a building block in organic synthesis, analgesics, and NSAIDs. You can find detailed UV spectra of Methoxyphenylacetic acid and information about its various lambda maxima by visiting the following link.
This application describes a normal-phase chiral HPLC method for the separation of Methoxyphenylacetic acid enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.
Methoxyphenylacetic acid can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.
Condition
| Column | Cyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Hexane/IPA/TFA- 70/30/0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 230 nm |
| Resolution | 5.94 |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Methoxyphenylacetic acid |
Application Column
Cyralis CM
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of Methoxyphenylacetic acid
February 26, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Methoxyphenylacetic acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
Separation of Methoxyphenylacetic acid on Newcrom R1 HPLC column
May 16, 2018
Methoxyphenylacetic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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