2’,4’,5’,7’-Tetrabromofluorescein

2’,4’,5’,7’-Tetrabromofluorescein structural formula

CAS Number15086-94-9
Molecular FormulaC20H8Br4O5
Molecular Weight647.896
InChI KeyDBZJJPROPLPMSN-UHFFFAOYSA-N
LogP5.80
Synonyms
  • 2',4',5',7'-Tetrabromofluorescein
  • 2',4',5',7'-Tetrabromo-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',4',5',7'-tetrabromo-3',6'-dihydroxy-
  • 15086-94-9
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',4',5',7'-tetrabromo-3',6'-dihydroxy-
  • 4-19-00-02917
  • C.I. 45380:2
  • D and C Red No. 21
  • BRN 0063410
  • Bromeosin
  • Bromoeosin
  • EINECS 239-138-3
  • Eosin acid
  • Eosine acid
  • Japan Red No. 223
  • NSC 244436
  • Red No. 223
  • Spiro(isobenzofuran-1(3H),9'-(9H)-xanthen)-3-one, 2',4',5',7'-tetrabromo-3',6'-dihydroxy-
  • Eosin Y spirit soluble
  • 3,6-Dihydroxy-2,4,5,7-tetrabromospiro(xanthene-9,3'-phthalide)
  • UNII-2730CL9N7C
  • D & C Red no. 21
  • Japan Red 223
  • Solvent Red 43
  • Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one,2',4',5',7'-tetrabromo-3',6'-dihydroxy-
  • 1043892-09-6
  • 113601-31-3
  • 19052-67-6
  • 30495-79-5
  • 342043-92-9
  • 477-63-4
  • 722454-16-2
  • 732983-53-8
  • 859629-60-0

Applications:

Separation of 2’,4’,5’,7’-Tetrabromofluorescein on Newcrom R1 HPLC column

February 16, 2018
Separation of 2’,4’,5’,7’-Tetrabromofluorescein on Newcrom C18 HPLC column

2’,4’,5’,7’-Tetrabromofluorescein can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
2’,4’,5’,7’-Tetrabromofluorescein
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.