Zwitterion

Synonyms

    Applications:

    Retention of Taurine on Obelisc N Column

    July 3, 2013

    Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the Obelisc N HILIC/mixed-mode column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.

    Condition

    Column Obelisc N, 4.6×50 mm, 5 µm, 100A
    Mobile Phase MeCN
    Buffer AmFm pH 3.0
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Taurine

     

    Application Column

    Obelisc N

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Taurine
    Zwitterion

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Retention of Taurine on SHARC Column

    July 3, 2013

    Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the SHARC 1 hydrogen-bonding column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.

    Condition

    Column Sharc 1, 4.6×50 mm, 5 µm, 100A
    Mobile Phase MeCN/MeOH
    Buffer Formic Acid – 0.1%, AmFm – 0.01%
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Taurine

     

    Application Column

    SHARC 1

    The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

    Select options
    Application Analytes:
    Taurine
    Zwitterion

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Glutamic Acid and GABA

    July 8, 2011

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    Glutamic acid and GABA are neurotransmitters. Glutamic acid and GABA are non-essential amino acids. They are hydrophilic and zwitter-ionic in nature . At lower pH, carboxylic acid groups of amino acids are not ionized, making them more hydrophobic and basic. Underivatized glutamic acid and GABA were retained and separated on a Primesep 100 column using ACN/water/TFA mobile phase. Amino acids can be monitored by low UV or ELSD/CAD. Retention is provided by reversed-phase and cation-exchange mechanism. Method can be used for analysis of underivatized amino acids in various matrices including supplements, vitamin and other complex mixtures. various mobile phase can be used with corresponding detection techniques.

    Condition

    Column Primesep 100, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 10/90%
    Buffer TFA – 0.1%
    Flow Rate 1.0 ml/min
    Detection UV, 215 nm, ELSD

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Glutamic acid, GABA

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Glutamic Acid
    Zwitterion
    gamma-Aminobutyric Acid (GABA)

    Application Detection:
    ELSD Detection
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Morpholino Sulfates

    November 21, 2010


    MOPS and MES are buffers used in biology and biochemistry. They consist of a morpholine ring and propanesulfonic acid. Both molecules are zwitter-ionic and very polar. No retention can be achieved on reversed-phase columns. MOPS and MES are analyzed in HILIC mode on a Primesep N HPLC column. Other biological buffers can be analyzed with this general method. Biological buffers are not UV active and can be monitored by ELSD, CAD or LC/MS.

    Application Column

    Primesep N

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    2-Morpholinoethanesulfonic Acid (MES)
    4-Morpholinepropanesulfonic Acid (MOPS)
    Zwitterion

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Comparison of Obelisc N, SeQuant?® HILIC, and Bare Silica

    March 3, 2010


    Obelisc N mixed-mode columns provide different selectivity than other HILIC columns — presence of ion-exchange mechanism contributes to a different selectivity. Depending on the pH of the mobile phase, ion-exchange mechanism can be enhanced or suppressed. Two amino acids (glycine and glycylglycine), two basic compounds (triethanolamine and TRIS) and two zwitter-ionic compounds (MOPS and MES) are separated by combination of HILIC and ion-exchange mechanisms. Compounds elution can be monitored by Evaporative Light-Scattering Detector (ELSD), Corona (CAD), LC/MS or other detection techniques.

    Application Column

    Obelisc N

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Zwitterion

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N

    March 3, 2010


    GABA (neurotransmitter) and its isomers are polar zwitter-ionic compounds. Due to the position of amino-groups, all three compounds show different polar and basic properties. The isomers of aminobuturic acid are separated on an Obelisc N HILIC/cation-exchange column. Buffer concentration has a different effect on retention of alpha-, beta-, and gamma-aminobutyric acid. This general and robust method can be used for separation of other polar and ionizable compounds and isomers by mixed-mode chromatography.

    Condition

    Column Obelisc N,  4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmFm pH 3.0
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Acid
    Analyzing Compounds Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA)

    Application Column

    Obelisc N

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Zwitterion
    alpha-Aminobutyric Acid
    beta-Aminobutyric Acid
    gamma-Aminobutyric Acid (GABA)

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Effect of Buffer on HPLC Separation of Buffers

    August 22, 2008

    HEPES, CAPS, MES and MOPS are zwitterionic organic chemical buffering agents. These are very polar compounds which are not retained by traditional reverse phase chromatography. These compounds are zwitterions in nature and can be separated by mixed-mode hydrophilic interaction chromatography on Obelisc N column. Retention is achieved by combination of HILIC and ion-exchange mechanisms. These buffering agents do not have UV active groups, but can be analyzed with ESLD, LC/MS, and CAD detection.

    Application Column

    Obelisc N

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    2-Morpholinoethanesulfonic Acid (MES)
    4-Morpholinepropanesulfonic Acid (MOPS)
    CAPS (3-(Cyclohexylamino)propanesulfonic acid)
    HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)
    Zwitterion

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Method for Analysis of Taurine on Primesep D Column

    December 6, 2007

    Taurine (2-aminoethanesulfonic acid) is organic zwitterionic compound. Taurine is a non-essential sulfur-containing amino acid that functions with glycine and gamma-aminobutyric acid as a neurotransmitter. Taurine is incorporated into one of the most abundant bile acids, chenodeoxycholic acid, where it serves to emulsify dietary lipids in the intestine, promoting digestion. It is used in food and pharmaceutical formulations. High polarity and zwitterionic nature of taurine complicates analysis of this compound by reverse-phase chromatography. Two methods for the analysis of taurine are developed on mixed-mode columns. Taurine is retained on Primesep A column by HILIC cation-exchange mechanism and on Primesep D column by HILIC anion-exchange mechanism. Method can be used for fast and effective quantitation of taurine in various products including energy drinks. Method requires ELSD or LC/MS detection due to lack of UV activity for taurine.

    Application Column

    Primesep D

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A

    Add to cart
    Application Analytes:
    Taurine
    Zwitterion

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Taurine on Primesep A

    December 6, 2007

    Taurine (2-aminoethanesulfonic acid) is organic zwitterionic compound. Taurine is a non-essential sulfur-containing amino acid that functions with glycine and gamma-aminobutyric acid as a neurotransmitter. Taurine is incorporated into one of the most abundant bile acids, chenodeoxycholic acid, where it serves to emulsify dietary lipids in the intestine, promoting digestion. It is used in food and pharmaceutical formulations. High polarity and zwitterionic nature of taurine complicates analysis of this compound by reverse-phase chromatography. Two methods for the analysis of taurine are developed on mixed-mode columns. Taurine is retained on Primesep A column by HILIC cation-exchange mechanism and on Primesep D column by HILIC anion-exchange mechanism. Method can be used for fast and effective quantitation of taurine in various products including energy drinks. Method requires ELSD or LC/MS detection due to lack of UV activity for taurine.

    Application Column

    Primesep A

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A

    Add to cart
    Application Analytes:
    Taurine
    Zwitterion

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Retention Characteristics of Betaine in Mixed-Mode HPLC

    May 5, 2005

    HPLC Application: Retention Characteristics of Betaine in Mixed-Mode HPLC

    The retention of the zwitterion betaine on Primesep 200 demonstrates the tunability of Primesep columns. Retention of betaine is altered by acid type, amount of acid in the mobile phase, and the amount of organic solvent. Typical reversed-phase columns do not show this tunability with simple mass spec compatible mobile phases of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).

    Application Column

    Primesep 200

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Betaine
    Quaternary Amines
    Zwitterion

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    TFA Concentration Effect on Retention of Tricine

    April 5, 2004

    Primesep A retains the hydrophilic zwitterion tricine by a combination of polar interactions and ion exchange. Tricine is not retained by traditional reversed-phase chromatography. The retention on Primesep A is adjustable by changing the concentration of TFA in the mobile phase. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).

    Application Column

    Primesep A

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Tricine
    Zwitterion

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.