Sulfanilamide

CAS Number63-74-1
Molecular FormulaC6H8N2O2S
Molecular Weight172.202
InChI KeyFDDDEECHVMSUSB-UHFFFAOYSA-N
LogP-0.6
Synonyms
  • sulfanilamide
  • 4-Aminobenzenesulfonamide
  • 63-74-1
  • Sulphanilamide
  • Sulfamine
  • Sulphonamide
  • p-Aminobenzenesulfonamide
  • Sulfonylamide
  • p-Aminobenzenesulfamide
  • Bacteramid
  • Streptasol
  • p-Sulfamoylaniline
  • Prontosil Album
  • Sulfonamide P
  • Estreptocida
  • Exoseptoplix
  • Streptoclase
  • p-Sulfamidoaniline
  • Streptocide
  • Sulfamidyl
  • Sulfanalone
  • Sulfanidyl
  • Sulfanil
  • Sulfocidine
  • Sulfana
  • Sulfanilimidic acid
  • p-Anilinesulfonamide
  • p-Aminophenylsulfonamide
  • Ambeside
  • Antistrept
  • Astreptine
  • Astrocid
  • Bactesid
  • Collomide
  • Colsulanyde
  • Copticide
  • Deseptyl
  • Ergaseptine
  • Erysipan
  • Gombardol
  • Lysococcine
  • Neococcyl
  • Orgaseptine
  • Prontalbin
  • Prontylin
  • Proseptal
  • Proseptine
  • Proseptol
  • Pysococcine
  • Septanilam
  • Septinal
  • Septolix
  • Septoplex
  • Septoplix
  • Strepamide
  • Strepsan
  • Streptagol
  • Streptamid
  • Streptamin
  • Streptocid
  • Streptocom
  • Strepton
  • Streptopan
  • Streptosil
  • Streptozol
  • Streptozone
  • Streptrocide
  • Sulfocidin
  • Therapol
  • 4-Sulfamoylaniline
  • Albexan
  • Albosal
  • Dipron
  • Gerison
  • Infepan
  • Sanamid
  • Stramid
  • Tolder
  • Benzenesulfonamide, 4-amino-
  • Lusil
  • 4-Aminobenzene-1-Sulfonamide
  • Prontosil White
  • Pronzin Album
  • Septamide Album
  • Stopton Album
  • Streptocid album
  • Prontosil I
  • Rubiazol A
  • White streptocide
  • PABS

Applications:

HPLC Separation of Antibiotics on Newcrom A Column

June 11, 2020


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Antibiotics are widely used for treatment and prevention of bacterial infections.  Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups.  Methanol can be used in the mobile phase on Newcrom A column.  The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer.  UV detection at 275nm.

Condition

Column Newcrom A, 3.2 x 100 mm, 5 µm, 100 A
Mobile Phase MeOH Gradient
Buffer H2SO4 Gradient
Flow Rate  0.5 ml/min
Detection UV 275 nm

 

Description

Class of Compounds Drugs, Antibiotics
Analyzing Compounds Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline

Application Column

Newcrom A

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Dapsone
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Antibiotics on Primesep 200 Column

June 6, 2020


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Antibiotics are widely used for treatment and prevention of bacterial infections.  Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups.  Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups.  In addition, methanol can be used in the mobile phase on Newcrom A column.  The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer.  UV detection at 275nm.

Condition

Column Primesep 200, 4.6 x 100 mm, 5 µm, 100 A
Mobile Phase MeCN Gradient
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 275 nm

 

Description

Class of Compounds Drugs, Antibiotics
Analyzing Compounds Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Dapsone
Sulfadiazine
Sulfaguanidine
Sulfanilamide

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Benzenesulfonamide and Sulfanilamide

August 22, 2008


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Condition 1

Column Newcrom А, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeOH/H2O – 80/20%
Buffer H2SO4 – 0.005%
Flow Rate 0.5 ml/min
Detection  275 nm


Condition 2

Column Primesep D, , Primesep 100 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer Formic Acid – 0.1%
Flow Rate 0.5 ml/min
Detection UV 230 nm

Both Benzenesulfonamide and sulfanilamide are organic antibacterial compounds differing only in the addition of an extra amino group in sulfanilamide. The closely related compounds can be separated on a mixed-mode Primesep D column by hydrophobic and anion-exchange mechanisms or Primesep 100 column by hydrophobic and cation-exchange mechanisms with the elution order reversed using identical mobile phases. UV Detection at 230nm.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Benzenesulfonamide
Sulfanilamide

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Aromatic Sulfonamides and Hydrozine

December 6, 2007

Application Analytes:
Benzenesulfonamide
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC

May 5, 2005

Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzenesulfonamide
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide
Sulfonamides

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.