| CAS Number | 63-74-1 |
|---|---|
| Molecular Formula | C6H8N2O2S |
| Molecular Weight | 172.202 |
| InChI Key | FDDDEECHVMSUSB-UHFFFAOYSA-N |
| LogP | -0.6 |
| Synonyms |
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Applications:
HPLC Separation of Antibiotics on Newcrom A Column
June 11, 2020
Antibiotics are widely used for treatment and prevention of bacterial infections. Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups. Methanol can be used in the mobile phase on Newcrom A column. The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer. UV detection at 275nm.
| Column | Newcrom A, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeOH Gradient |
| Buffer | H2SO4 Gradient |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Drugs, Antibiotics |
| Analyzing Compounds | Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline |
Application Column
Newcrom A
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline
HPLC Separation of Antibiotics on Primesep 200 Column
June 6, 2020
Antibiotics are widely used for treatment and prevention of bacterial infections. Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups. Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups. In addition, methanol can be used in the mobile phase on Newcrom A column. The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer. UV detection at 275nm.
| Column | Primesep 200, 4.6 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN Gradient |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Drugs, Antibiotics |
| Analyzing Compounds | Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Sulfadiazine
Sulfaguanidine
Sulfanilamide
HPLC Separation of Benzenesulfonamide and Sulfanilamide
August 22, 2008
| Column | Newcrom А, 3.2×50 mm, 3 µm, 100A |
| Mobile Phase | MeOH/H2O – 80/20% |
| Buffer | H2SO4 – 0.005% |
| Flow Rate | 0.5 ml/min |
| Detection | 275 nm |
| Column | Primesep D, , Primesep 100 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | Formic Acid – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 230 nm |
Both Benzenesulfonamide and sulfanilamide are organic antibacterial compounds differing only in the addition of an extra amino group in sulfanilamide. The closely related compounds can be separated on a mixed-mode Primesep D column by hydrophobic and anion-exchange mechanisms or Primesep 100 column by hydrophobic and cation-exchange mechanisms with the elution order reversed using identical mobile phases. UV Detection at 230nm.
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSulfanilamide
HPLC Separation of Aromatic Sulfonamides and Hydrozine
December 6, 2007
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide
Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC
May 5, 2005
Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsChlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide
Sulfonamides
