| CAS Number | 7681-93-8 |
|---|---|
| Molecular Formula | C33H47NO13 |
| Molecular Weight | 665.7 |
| InChI Key | NCXMLFZGDNKEPB-FFPOYIOWSA-N |
| LogP | -1.3 |
| Synonyms |
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Applications:
HPLC UV Method for Analysis of Natamycin in Yogurt on Primesep 100 Column
July 13, 2026
HPLC Method for Analysis of Natamycin on Primesep 100 Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Natamycin.
Natamycin, also known as pimaricin, is an antifungal medication with C33H47NO13 molecular formula. It is primarily used to treat fungal infections of eyelids, conjunctiva, and cornea. On occasion, it is also used as a preservative. It prevents fungi growth by binding to ergosterol in fungal cell membranes and inhibiting amino acid and glucose transport proteins. You can find detailed UV spectra of Natamycin and information about its various lambda maxima by visiting the following link.
**The Yogurt sample was prepared by mixing 5g of yogurt and 5g or acetonitrile. After the mixture separated, the liquid half was removed and filtered.
Natamycin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN/H2O 10/90 to 80/20% over 20 min |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 320 nm |
| Limit Of Detection* | Natamycin – 0.2 ppb |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Natamycin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Analysis of Natamycin on Primesep 100 Column
July 13, 2026
HPLC Method for Analysis of Natamycin on Primesep 100 Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Natamycin.
Natamycin, also known as pimaricin, is an antifungal medication with C33H47NO13 molecular formula. It is primarily used to treat fungal infections of eyelids, conjunctiva, and cornea. On occasion, it is also used as a preservative. It prevents fungi growth by binding to ergosterol in fungal cell membranes and inhibiting amino acid and glucose transport proteins. You can find detailed UV spectra of Natamycin and information about its various lambda maxima by visiting the following link.
Natamycin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O- 40/60% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 320 nm |
| Limit Of Detection* | 0.2 ppb |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Natamycin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of Natamycin
July 13, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Natamycin check our HPLC Applications library
UV/Vis.: λmax: 220, 293, 304, 320 nm
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in ethanol. This analysis was done with the mobile phase of hexane, IPA, and TFA.
This compound was diluted in a mixture of water and acetonitrile. The method was done with a mixture of water, acetonitrile, and a sulfuric acid buffer.
