| CAS Number | 521-31-3 |
|---|---|
| Molecular Formula | C8H7N3O2 |
| Molecular Weight | 177.163 |
| InChI Key | HWYHZTIRURJOHG-UHFFFAOYSA-N |
| LogP | 0.192 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Luminol on Primesep 100 Column
July 15, 2026
HPLC Method for Analysis of Luminol on Primesep 100 Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Luminol.
Luminol is a compound with the chemical formula C8H7N3O2. It is primarily known for exhibiting chemiluminescence, glowing blue when mixed with an oxidizing agent. It has been used in criminal investigation since the 1930s due to it’s luminescence being enhanced when mixed with blood. Due to the small amount of iron needed as a catalyst, it shows traces of blood even when blood has been cleaned or removed. You can find detailed UV spectra of Luminol and information about its various lambda maxima by visiting the following link.
Luminol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and water. Detection is performed using UV.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 354 nm |
| Limit Of Detection* | 90 ppb |
Description
| Class of Compounds | Hydrazide |
| Analyzing Compounds | Luminol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of Luminol
July 14, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Luminol check our HPLC Applications library
UV/Vis.: λmax: 218, 293, 354 nm
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in ethanol. This analysis was done with the mobile phase of hexane, IPA, and TFA.
This compound was diluted in a mixture of water and acetonitrile. The method was done with a mixture of water, acetonitrile, and a sulfuric acid buffer.
Separation of Luminol on Newcrom R1 HPLC column
February 16, 2018
Luminol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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