HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH

HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH Column_1245

Uracil, Thymine, Guanine, Cytosine and Adenine are the nucleobases found in RNA and DNA.  The nucleobases are difficult to separate on reverse-phase columns due to their polar, hydrophilic and ionic nature.  Using the Newcrom AH mixed-mode column, the nucleobases can be easily separated isocratically using low organic mobile phase (5% acetonitrile) or pure water, if organic mobile phase is undesirable, with ammonium formate buffer, making the method both UV and Mass Spec compatible.

ColumnNewcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 5/95%
BufferAmFm pH 3.0- 30 mM
Flow Rate1.0 ml/min
DetectionUV 260 nm,  MS-compatible mobile phase

ColumnNewcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile PhaseH2O – 100%
BufferAmFm pH 3.0- 10 mM
Flow Rate1.0 ml/min
DetectionUV 260 nm,  MS-compatible mobile phase

Class of CompoundsHydrophilic, Drug, Xanthine, Nucleic Bases
Analyzing CompoundsUracil, Thymine, Guanine, Cytosine, Adenine

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Adenine
Cytosine
Guanine
Thymine
Uracil
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.