HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH

HPLC.cloud View on hplc.cloud HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH Column_1245 Uracil, Thymine, Guanine, Cytosine and Adenine are the nucleobases found in RNA and DNA.  The nucleobases are difficult to separate on reverse-phase columns due to their polar, hydrophilic and ionic nature.  Using the Newcrom AH mixed-mode column, the nucleobases can be easily separated isocratically using low organic mobile phase (5% acetonitrile) or pure water, if organic mobile phase is undesirable, with ammonium formate buffer, making the method both UV and Mass Spec compatible.

Condition 1

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm,  MS-compatible mobile phase

Condition 2

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase H2O - 100%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm,  MS-compatible mobile phase

Description

Class of Compounds Hydrophilic, Drug, Xanthine, Nucleic Bases
Analyzing Compounds Uracil, Thymine, Guanine, Cytosine, Adenine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Application Analytes:
Adenine
Cytosine
Guanine
Thymine
Uracil