HPLC Chiral Separation of Benzoin Enantiomers on Cyralis CM Column

HPLC Method for Analysis of Benzoin Enantiomers on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzoin.

Benzoin is an organic compound with the chemical formula C14H12O2. It is primarily used as a precursor to Benzil or in powder coating. You can find detailed UV spectra of Benzoin and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Benzoin enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Benzoin can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and Ethanol. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol – 70/30
Flow Rate1.0 mL/min
DetectionUV 250 nm
Resolution3.70

Description

Class of CompoundsOrganic Compound
Analyzing CompoundsBenzoin

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzoin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.