HPLC Method For Analysis Of 2,4,6-Trichloroanisole on Newcrom R1 Column on Alltesta™

HPLC Method for 2,4,6-Trichloroanisole on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2,4,6-Trichloroanisole.

2,4,6-Trichloroanisole (TCA) is a fungal metabolite of 2,4,6-Trichlorophenol with the chemical formula C7H5Cl3O. TCA has a distinct musty smell that is most evident when wines develop cork taint. It is formed by the methylation of 2,4,6-trichloropenol.  Naturally, it is suspected to be produced when airborne fungi and bacteria are presented with chlorinated phenolic compounds. You can find detailed UV spectra of 2,4,6-Trichloroanisole and information about its various lambda maxima by visiting the following link.

2,4,6-Trichloroanisole can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

Condition
Column Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 70/30%
Buffer None
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Haloanisole
Analyzing Compounds 2,4,6-Trichloroanisole

 

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
2,4,6-Trichloroanisole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.