HPLC Method for Lactic Acid, Lactic Acid Oligomers on Newcrom BH by SIELC Technologies
Lactic acid (milk acid) is a natural organic acid that plays an important role in biochemical processes. It has a carboxylic and hydroxy- groups which, upon standing, can react with each other and form dimers, trimers and higher oligomers. Lactic acid is a polar molecule that does not retain on reversed-phase columns. Newcrom BH mixed-mode anion-exchange column is used for the separation of lactic acid and its oligomers by a combination of weak reversed-phase and anion-exchange mechanisms. Lactates can be monitored by low UV, ELSD, CAD or LC/MS.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O |
| Buffer | HClO4 – 0.1% |
| Flow Rate | 1 ml/min |
| Detection | UV 210 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Lactic Acid, Lactic Acid Oligomers |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Lactic AcidLactic Acid Oligomers
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
