HPLC Chiral Separation of Tramadol Enantiomers on Cyralis CM Column

HPLC Method for Analysis of Tramadol on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tramadol.

Tramadol is a narcotic analgesic with the chemical formula C16H25NO2. It is used to manage severe pain. It is a synthetic codeine analogue. The way it works is similar to opioids, such as morphine and codeine due to it acting on specific opioid receptors. An overdose on the drug can cause acute liver failure. Tramadol, also known as Ultram and other brand names, is a synthetic opiate used to treat moderate to severe pain. The painkilling effects of Tramadol last for about 6 hours. You can find detailed UV spectra of Tramadol and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Tramadol enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Tramadol can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and 2-(dimethylamino)ethanol. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA/2-(dimethylamino)ethanol
Flow Rate1.0 mL/min
DetectionUV 270 nm
Resolution1.64

Description

Class of CompoundsDrug
Analyzing CompoundsTramadol

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Tramadol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.