HPLC Chiral Separation of Pindolol on Cyralis CM Column

HPLC Method for Analysis of Pindolol on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pindolol.

Pindolol is a beta-blocker with the chemical formula C14H20N2O2. It is primarily used to treat hypertension as well as an antagonist of the serotonin 5-HT1A receptor. Due to the Coronavirus outbreak, Pindolol is on the FDA Drug Shortage list. You can find detailed UV spectra of Pindolol and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Pindolol enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Pindolol can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Ethanol and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseEthanol/DEA- 99.7/0.3%
Flow Rate1.0 mL/min
DetectionUV 250 nm
Resolution4.45

Description

Class of CompoundsBeta-Blocker
Analyzing CompoundsPindolol

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Pindolol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.