HPLC Chiral Separation of Ibuprofen on Cyralis CM Column

HPLC Method for Analysis of Ibuprofen on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ibuprofen.

Ibuprofen is a nonsteroidal anti-inflammatory drug (NSAID) with the chemical formula C13H18O2. It is used primarily to relieve pain, fever, and inflammation. There is numerous adverse effects to taking ibuprofen, including but not limited to nausea, heightened risk of hypertension, kidney impairment, and bronchospasm. You can find detailed UV spectra of Ibuprofen and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Ibuprofen enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Ibuprofen can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and TFA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol/TFA- 99/1/0.02%
Flow Rate0.75 mL/min
DetectionUV 230 nm
Resolution1.72

Description

Class of CompoundsMedication
Analyzing CompoundsIbuprofen

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ibuprofen
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.