Diphenylamine

Diphenylamine structural formula

CAS Number122-39-4
Molecular FormulaC12H11N
Molecular Weight169.228
InChI KeyDMBHHRLKUKUOEG-UHFFFAOYSA-N
LogP3.50
Synonyms
  • Diphenylamine
  • N-Phenylaniline
  • Benzenamine, N-phenyl-
  • 122-39-4
  • N-Phenylbenzenamine
  • Benzenamine, N-phenyl-
  • Amino diphenyl
  • Anilinobenzene
  • Benzene, (phenylamino)-
  • difenilamina
  • Diphenylamin
  • DIPHENYLAMIN, REIN
  • DIPHENYLAMIN, TECHNISCH
  • Diphenylimine
  • N,N-Diphenylamine
  • Naugalube 428L
  • No-Scald
  • N-PHENYL-ANILIN
  • N-Phenylaniline
  • N-PHENYLBENZENEAMINE
  • NSC 215210
  • Aniline, N-phenyl-
  • Benzene, anilino-
  • Caswell No. 398
  • EINECS 204-539-4
  • EPA Pesticide Chemical Code 038501
  • UNII-9N3CBB0BIQ
  • (phenylamino)-Benzene
  • (phenylamino)benzene
  • 2-Biphenylyl-N-pyridyl-Acetamide
  • 2-Biphenylyl-N-pyridylacetamide
  • Anilino-Benzene
  • Benzenamine, N-phenyl-, styrenated
  • Big dipper
  • C6H5-NH-C6H5
  • DFA
  • DPA
  • Deccoscald 282
  • Difenylamin
  • Diphenpyramide
  • Diphenyl-amine
  • Diphenylamine indicator
  • Diphenylamine, acs
  • Diphenylamine, reaction product with 2,2,4-trimethylpentene
  • N-Fenylanilin
  • N-Phenyl-Aniline
  • N-Phenyl-Benzenamine
  • N-Phenylbenzenamine, 9CI
  • N-Phenylbenzenamine, styrenated
  • No scald
  • No scald dpa 283
  • No-Scald DPA 283
  • Phenylaniline
  • Poly(diphenylamine)
  • Pyridyl-biphenylyl-acetamide
  • Scaldip
  • Shield dpa
  • Styrenated diphenylamine
  • Styrene, reaction product with diphenylamine
  • 1135443-74-1

Applications:

HPLC Method for Analysis of Diphenylamine on Newcrom R1 Column on Alltesta™

June 23, 2026

HPLC Method for Diphenylamine on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Diphenylamine 

Diphenylamine is an organic compound with the chemical formula (C6H5)2NH. It is used primarily to distinguish DNA from RNA with the Dsiche test, as a scald inhibitor in apples, antioxidant, stabilizer of smokeless powder, and a redox indicator. It’s a derivative of aniline, while Metanil Yellow, Disperse Orange 1, and Acid Orange 5 are derivatives of Diphenylamine. You can find detailed UV spectra of Diphenylamine and information about its various lambda maxima by visiting the following link.

Diphenylamine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnNewcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN -60/40%
BufferH3PO4 – 0.1%
Flow Rate0.5 mL/min
DetectionUV 275 nm
Class of CompoundsOrganic Compound
Analyzing CompoundsDiphenylamine

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Diphenylamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Uv-Vis Spectrum of Diphenylamine

March 26, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Diphenylamine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in Acetonitrile.

Application Analytes:
Diphenylamine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Diphenylamine on Newcrom R1 HPLC column

February 16, 2018
Separation of Diphenylamine on Newcrom C18 HPLC column

Diphenylamine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Diphenylamine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.