N,N'-Bis(3-aminopropyl)-1,3-propanediamine

CAS Number	4605-14-5
Molecular Formula	C9H24N4
Molecular Weight	188.320
InChI Key	ZAXCZCOUDLENMH-UHFFFAOYSA-N
LogP	-0.665
Synonyms	1,3-Propanediamine, N,N'-bis(3-aminopropyl)- N¹,N~1'~-(Propane-1,3-diyl)di(propane-1,3-diamine) 1,3-Propanediamine, N¹,N³-bis(3-aminopropyl)- 4605-14-5 EINECS 225-007-8

Applications:

HPLC Separation of Polyamines on Primesep 500 Column

2020-06-26

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Polyamines are both naturally occurring and synthetic. The naturally occurring polyamines have a variety of functions including ion channel modulation and biosynthesis of cells. The synthetic polyamines are used in motor oil and epoxy resins. Because of their strong ionic characteristics, they are not retained on reverse-phase columns and strongly retained on anion-exchange columns. In order to separate polyamines with varying numbers of ionic groups, we used a Primesep 500 HPLC mixed-mode column with embedded carboxylic groups of pKa around 5. By lowering the pH of the mobile phase, we can suppress the carboxylic groups on the column making it less retentive to polyamines. We separate the double and triple charged polyamines at one pH, then do a step gradient to lower pH to reduce the retention time and separate higher charged polyamines. The detection can be achieved with CAD or MS.

Condition
Column	Primesep 500, 4.6×150 mm, 5 µm, 100A
Mobile Phase	H2O
Buffer	AmFm pH 3.0 – 20 mM, AmFm pH 3.5 – 20 mM
Flow Rate	1.0 ml/min
Detection	CAD

Description
Class of Compounds	Hydrophilic, Amines
Analyzing Compounds	Ethylenediamine, N-(3-Aminopropyl)-1,3-propanediamine, N,N’- Bis(3-aminopropyl)-1,3-propanediamine, Tetraethylenepentamine, Pentaethylenehexamine

Application Column

Primesep 500

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options

Ethylenediamine
N,N’-Bis(3-aminopropyl)-1,3-propanediamine
N-(3-Aminopropyl)-1,3-propanediamine (Norspermidine)
Pentaethylenehexamine
Polyamines
Tetraethylenepentamine

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Polyamines

2004-04-13

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Primesep 200 separates the very hydrophilic polyamines, 1,3-diaminopropane, N-(3-aminopropyl)-1,3-diaminepropane, N,N-bis-(3-aminopropyl)-1,3-diaminepropane. The retention and resolution are affected by either a TFA concentration gradient, or a pH gradient from pH 4 to 3. This flexibility is obtained by simple mobile phases of water, acetonitrile (MeCN, ACN), ammonium formate, ammonium acetate or trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).