| CAS Number | 88040-23-7 |
|---|---|
| Molecular Formula | C19H24N6O5S2 |
| Molecular Weight | 480.560 |
| InChI Key | HVFLCNVBZFFHBT-ZKDACBOMSA-N |
| LogP | 1.40 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Cefepime
January 14, 2026
If you are looking for optimized HPLC method to analyze Cefepime check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Determination of Cefepime on Primesep 100 Column
August 5, 2021
HPLC Method for Cefepime on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis Cefepime
Cefepime is a fourth generation cephalosporin antibiotic usually reserved for treatment of severe nosocomial pneumonia and febrile neutropenia as well as multiple drug-resistant microorganisms such as Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae.
Cefepime can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 270 nm.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Cefepime |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Analysis of Cefepime
August 6, 2015
Cefepime is a fourth generation cephalosporin antibiotic usually reserved to treat severe nosocomial pneumonia and febrile neutropenia as well as multiple drug-resistant microorganisms such as Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. Legacy L1 was used to retain Cefepime by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.
| Column | Legacy L1, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeOH – 10% |
| Buffer | NaH2PO4 pH 5.0- 5 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable |
| Analyzing Compounds | Cefepime |
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
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