Pyridoxal Phosphate

CAS Number54-47-7
Molecular FormulaC8H10NO6P
Molecular Weight247.14
InChI KeyNGVDGCNFYWLIFO-UHFFFAOYSA-N
LogP-1.1
Synonyms
  • pyridoxal phosphate
  • Codecarboxylase
  • pyridoxal 5-phosphate
  • 54-47-7
  • pyridoxal 5'-phosphate
  • Pyridoxal P
  • Biosechs
  • Hairoxal
  • Pyromijin
  • Vitazechs
  • Hiadelon
  • Pidopidon
  • Himitan
  • Phosphopyridoxal
  • Pyridoxal-5'-phosphate
  • Pal-P
  • Sechvitan
  • Pydoxal
  • Piodel
  • Pyridoxyl phosphate
  • Apolon B6
  • HI-Pyridoxin
  • (4-formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate
  • PYRIDOXAL-5-PHOSPHATE
  • pyridoxal-P
  • Phosphopyridoxal coenzyme
  • Vitahexin P
  • Hexermin P
  • Coenzyme B6
  • Pyridoxal monophosphate
  • Pyridoxaldehyde phosphate
  • Apolon B(sub 6)
  • PLP
  • Phosphoridoxal coenzyme
  • 853645-22-4
  • pyridoxal-5P
  • Pyridoxal 5'-(dihydrogen phosphate)
  • Pyrido
  • Vitamin B6 phosphate
  • Vitahexin-P
  • Hexermin-P
  • Pyridoxal 5-monophosphoric acid ester
  • Pyridoxal phosphate [JAN]
  • Pyridoxal 5'-phosphate hydrate
  • UNII-F06SGE49M6
  • 4-Pyridinecarboxaldehyde, 3-hydroxy-2-methyl-5-((phosphonooxy)methyl)-
  • 3-Hydroxy-2-methyl-5-((phosphonooxy)methyl)-4-pyridinecarboxaldehyde
  • EINECS 200-208-3
  • Pyridoxal, 5-(dihydrogen phosphate)
  • NSC 82388
  • 3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinecarboxaldehyde
  • 2-Methyl-3-hydroxy-4-formyl-5-hydroxymethylpyridine-5-calcium phosphate
  • 4-pyridinecarboxaldehyde, 3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-
  • CHEMBL82202
  • F06SGE49M6
  • CHEBI:18405
  • NGVDGCNFYWLIFO-UHFFFAOYSA-N
  • 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde 5-phosphate
  • NSC82388
  • AK103579
  • (4-Formyl-5-hydroxy-6-methyl(3-pyridyl))methyl dihydrogen phosphate
  • 2-Methyl-3-hydroxy-4-formyl-5-hydroxymethylpyridine-5-calcium phosphate trihydrate
  • Q-201645
  • Pyridoxal calcium phosphate
  • (4-formyl-5-hydroxy-6-methyl-3-pyridyl)methyl dihydrogen phosphate
  • [(4-formyl-5-hydroxy-6-methylpyridin-3-yl)methoxy]phosphonic acid
  • 3-Hydroxy-2-methyl-5-([phosphonooxy]methyl)-4-pyridinecarboxaldehyde
  • Pyridoxalphosphate
  • Sechvitan, Vitahexin P
  • NCGC00166300-01
  • Pyridoxal-5-monophosphate
  • VITAMIN B6 COMPLEX
  • Pyridoxal 5'-phosphate 5
  • Pyridoxal phosphate (6CI)
  • C8H10NO6P
  • Pyridoxal-5-Phosphate Hydrate
  • Vitamin B6 phosphate (ester)
  • pyridoxal-phosphate
  • Pyridoxal, 5-(dihydrogenphosphate)
  • SRI 2392
  • P-5'-P
  • Pridoxal-5-Phosphate
  • pyridoxal 5''-phosphate
  • P5P
  • Pyridoxal, 5-(dihydrogen phosphate) (8CI)
  • bmse000111
  • Pyridoxal 5'-phosphate monohydrate, vitamin B6
  • AC1L1AM8
  • AC1Q2P2R
  • Pyridoxal phosphate anhydrous
  • SCHEMBL23158
  • Pyridoxal 5'-phosphoric acid
  • KSC269K4B
  • GTPL5249
  • SGCUT00188
  • DTXSID4048351
  • CTK1G9540
  • KS-00000QDE
  • Pyridoxal 5'-phosphate anhydrous
  • 2-Methyl-3-hydroxy-4-formyl-5-pyridylmethylphosphoric acid
  • EX-A980
  • 4-Formyl-5-hydroxy-6-methyl-pyridin-3-yl)methoxyphosphonic acid
  • ACT03489
  • HY-B1744
  • Pyridoxal phosphate treated .beta.-lactoglobulin from bovine whey
  • to_000077
  • ZINC1532514
  • (4-formyl-5-hydroxy-6-methyl-3-pyridinyl)methyl dihydrogen phosphate
  • ANW-64668
  • BDBM50118216
  • CP-968
  • MFCD00006333
  • NSC-82388
  • SBB065295
  • STL185213
  • pyridoxal 5''-(dihydrogen phosphate)
  • AKOS015891654
  • Phosphoric acid mono-(4-formyl-5-hydroxy-6-methyl-pyridin-3-ylmethyl) ester
  • API0003990
  • CS-7767
  • DB00114
  • MCULE-8936350631
  • SB18794
  • VZ33680
  • Pyridoxal 5'-phosphate hydrate, >=98%
  • AJ-26770
  • AN-24419
  • AN-27091
  • AS-19314
  • BC200450
  • SC-65250
  • SC-98887
  • AX8235118
  • LS-134383
  • TC-153413
  • TL8005582
  • TR-016430
  • FT-0631236
  • FT-0655876
  • Isonicotinaldehyde, 5-(dihydrogen phosphate)
  • ST24020566
  • X6777
  • A-7859
  • C00018
  • Pyridoxal 5'-phosphate monohydrate - Vitamin B6
  • 123869-EP2295417A1
  • 123869-EP2295439A1
  • 468C251
  • A841303
  • SR-01000944534
  • SR-01000944534-1
  • A26BDB6A-282A-4D13-A916-7B2B215B0FD6

Applications:

HPLC Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 Column

August 28, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate

Water-Soluble Vitamins (B1, B2, B3, B6) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 300 nm.

You can find detailed UV spectra of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate and information about its various lambda maxima by visiting the following links: Vitamins (B1, B2, B3, B6)

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 10%
BufferGradient H2SO4 -0.1-0.5% in 10 min
Flow Rate1.0 ml/min
DetectionUV 270 nm

Class of Compounds
Water-Soluble Vitamins (B1, B2, B3, B6)
Analyzing CompoundsVitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Nicotinamide
Pyridoxal Phosphate
Pyridoxamine
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Thiamine Pyrophosphate and Pyridoxal Phosphate on Primesep SB Column

July 11, 2017

Condition
Column Primesep SB, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 290 nm

 

Description
Class of Compounds
 Hydrophilic, Ionizable
Analyzing Compounds Thiamine Pyrophosphate, Pyridoxal Phosphate

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Pyridoxal Phosphate
Thiamine diphosphate (Thiamine pyrophosphate)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.