Thalidomide

Thalidomide structural formula

CAS Number50-35-1
Molecular FormulaC13H10N2O4
Molecular Weight258.233
InChI KeyUEJJHQNACJXSKW-UHFFFAOYSA-N
LogP0.33
Synonyms
  • Thalidomide
  • 2-(2,6-Dioxopiperidin-3-yl)-1H-isoindole-1,3(2H)-dione
  • 1H-Isoindole-1,3(2H)-dione, 2-(2,6-dioxo-3-piperidinyl)-
  • 50-35-1
  • 1H-Isoindole-1,3(2H)-dione, 2-(2,6-dioxo-3-piperidinyl)-
  • (.+-.)-Thalidomide
  • 1,3-Dioxo-2-(2,6-dioxopiperidin-3-yl)isoindoline
  • 3-Phthalimidoglutarimide
  • Celgene
  • Contergan
  • Distaval
  • Kevadon
  • N-(2,6-Dioxo-3-piperidyl)phthalimide
  • Neurosedyn
  • N-Phthaloylglutamimide
  • NSC 527179
  • NSC 66847
  • Pantosediv
  • Phthalimide, N-(2,6-dioxo-3-piperidyl)-
  • Quetimid
  • Sauramide
  • Sedalis
  • Sedoval
  • Softenil
  • Softenon
  • Suaramide
  • talidomida
  • Talimol
  • Talinol
  • Thalidomid
  • Thalomid
  • ?-(N-Phthalimido)glutarimide
  • ?-N-Phthalylglutaramide
  • ?-Phthalimidoglutarimide
  • Algosediv
  • Asmadion
  • Asmaval
  • Bonbrain
  • BRN 0030233
  • Calmore
  • Calmorex
  • Corronarobetin
  • 2,6-Dioxo-3-phthalimidopiperidine
  • Ectiluran
  • EINECS 200-031-1
  • Enterosediv
  • Gastrinide
  • Glutanon
  • Glutarimide, 2-phthalimido-
  • Grippex
  • Hippuzon
  • Imida-lab
  • Imidene
  • Neaufatin
  • Neosedyn
  • Neosydyn
  • Nerosedyn
  • Neufatin
  • Neurodyn
  • Neurosedin
  • Neurosedym
  • Nevrodyn
  • Noctosediv
  • Noxodyn
  • NSC-66847
  • alpha-Phthalimidoglutarimide
  • alpha-(N-Phthalimido)glutarimide
  • N-Phthalylglutamic acid imide
  • alpha-N-Phthalylglutaramide
  • Poly-giron
  • Polygripan
  • Predni-sediv
  • Pro-Bam M
  • Pro-ban M
  • Profarmil
  • Psycholiquid
  • Psychotablets
  • Quietoplex
  • Sandormin
  • Sedalis sedi-lab
  • Sedimide
  • Sedisperil
  • Shin-naito S
  • Shinnibrol
  • Sleepan
  • Talargan
  • Talismol
  • Telagan
  • Telargean
  • Tensival
  • Thalinette
  • Theophilcholine
  • Valgraine
  • Yodomin
  • Thalomide
  • (+-)-Thalidomide
  • N-Phthalyl-glutaminsaeure-imid
  • Talidomide
  • Thalidomidum
  • UNII-4Z8R6ORS6L
  • Thalidomide Celgene
  • 2-(2,6-dioxopiperidin-3-yl)-1H-isoindole-1,3(2H)-dione

Applications:

HPLC Chiral Separation of Thalidomide on Cyralis CM Column

June 19, 2026

HPLC Method for Analysis of Thalidomide on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Thalidomide.

Thalidomide is a an medication with the chemical formula C13H10N2O4. It is primarily used to treat cancer, graft-versus-host disease, and various skin disorders. Thalidomide can cause birth defects, which was a fact originally unknown when it entered the German market in 1957. Over 10,000 children were harmed by Thalidomide, which has led to stricter drug regulation and monitoring in many countries.

This application describes a normal-phase chiral HPLC method for the separation of Thalidomide enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Thalidomide can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and IPA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA- 50/50%
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution2.23

Description

Class of CompoundsMedication
Analyzing CompoundsThalidomide

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Thalidomide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Thalidomide on Newcrom R1 HPLC column

May 16, 2018
Separation of Thalidomide on Newcrom R1 HPLC column

Thalidomide can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Thalidomide
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.