| CAS Number | 5138-18-1 |
|---|---|
| Molecular Formula | C4H6O7S |
| Molecular Weight | 198.150 |
| InChI Key | ULUAUXLGCMPNKK-UHFFFAOYSA-N |
| LogP | -1.66 |
| Synonyms |
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Applications:
HPLC Determination of Sulfosuccinic Acid on Newcrom BH Column
August 6, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfosuccinic Acid.
Sulfosuccinic acid is a surfactant used in cosmetics and pharmaceuticals. Sulfosuccinic acid can be retained on the Newcrom BH mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
| Column | Newcrom BH, 3.2×100 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O -10/90% |
| Buffer | H2SO4 – 0.3% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Acid |
| Analyzing Compounds | Sulfosuccinic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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The Separation of Sodium, Dioctylsulfosuccinic Acid, and Sulfosuccinic Acid
July 2, 2013
Sulfosuccinic acid and the corresponding dioctyl ester were separated on the Primesep B4 short carbon-chain anion-exchange column. Short carbon chain stationary phase allows to elute hydrophobic and acidic diester in reasonable time. No elution was observed on the mixed-mode columns with longer carbon chain analogs due to very strong hydrophobic and anion-exchange interaction.
| Column | Primesep B4, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10 -50%, 10 min |
| Buffer | Gradient AmFm pH 3.0- 60-180 mM, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Dioctyl Sulfosuccinic acid, Sodium Docusate |
Application Column
Primesep B4
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSodium
Sulfosuccinic acid
HPLC Separation of Sodium, Dioctyl Sulfosuccinic Acid, and Sulfosuccinic Acid
July 10, 2012
Application Notes: Dioctyl sodium sulfosuccinate is a common component of consumer products and medications. It is also used as emulsifier, wetting, and dispersing agent. It is a highly acidic, and hydrophobic compound. It serves as a starting material for dioctyl sodium sulfonate. Both compounds were retained and separated on Primesep B4 mixed-mode reversed-phase anion-exchange column. The primesep B4 column has C4 carbon chain and it is much less hydrophobic than Primesep D or Primesep B2 column. The low hydrophobicity of the stationary phase is strong enough to provide reversed-phase retention for dioctyl sulfosuccinic acid. This method can be used for quantification of these two compounds in reaction mixtures and formulation. The method is LC/MS and ELSD/CAD compatible.
Application Columns: Primesep B4
Application compounds: Sodium, Dioctyl Sulfosuccinic Acid, Sulfosuccinic Acid
Detection technique: LC/MS, ELSD/CAD
| Column | Primesep B4, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10 -50%, 10 min |
| Buffer | Gradient AmFm pH 3.0- 60-180 mM, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Dioctyl Sulfosuccinic Acid, Sulfosuccinic Acid |
Application Column
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Primesep B4
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsDocusate sodium
Sodium
Sulfosuccinic acid
HPLC Analysis of Sulfosuccinic Acid on a Primesep S Column
July 10, 2012
Application Notes: Sulfosuccinic acid was retained on Primesep S HILIC/anion exclusion Primesep S column. Retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Since the compound has no UV activity ELSD was used for monitoring and quantitation.
Application Columns: Primesep S
Application compounds: Sulfosuccinic Acid
Detection technique: UV, LC/MS, ELSD/CAD
| Column | Primesep S, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sulfosuccinic Acid |
Application Column
Primesep S
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsUV Detection
