The Separation of Sodium, Dioctylsulfosuccinic Acid, and Sulfosuccinic Acid


Sulfosuccinic acid and the corresponding dioctyl ester were separated on the Primesep B4 short carbon-chain anion-exchange column. Short carbon chain stationary phase allows to elute hydrophobic and acidic diester in reasonable time. No elution was observed on the mixed-mode columns with longer carbon chain analogs due to very strong hydrophobic and anion-exchange interaction.



Column Primesep B4, 3.2×50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10 -50%, 10 min
Buffer Gradient AmFm pH 3.0- 60-180 mM, 10 min
Flow Rate 1.0 ml/min
Detection ELSD, 50C



Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Dioctyl Sulfosuccinic acid, Sodium Docusate


Application Column

Primesep B4

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Dioctyl sulfosuccinic acid
Sulfosuccinic acid

Application Detection:
ELSD Detection
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