Sulfosuccinic acid and the corresponding dioctyl ester were separated on the Primesep B4 short carbon-chain anion-exchange column. Short carbon chain stationary phase allows to elute hydrophobic and acidic diester in reasonable time. No elution was observed on the mixed-mode columns with longer carbon chain analogs due to very strong hydrophobic and anion-exchange interaction.
| Column | Primesep B4, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10 -50%, 10 min |
| Buffer | Gradient AmFm pH 3.0- 60-180 mM, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Dioctyl Sulfosuccinic acid, Sodium Docusate |
Application Column
Primesep B4
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSodium
Sulfosuccinic acid
