Application Notes: Sulfosuccinic acid was retained on Primesep S HILIC/anion exclusion Primesep S column. Retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Since the compound has no UV activity ELSD was used for monitoring and quantitation.
Application Columns: Primesep S
Application compounds: Sulfosuccinic Acid
Detection technique: UV, LC/MS, ELSD/CAD
Column | Primesep S, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | AmAc |
Flow Rate | 1.0 ml/min |
Detection | ELSD |
Class of Compounds |
Acid, Hydrophilic, Ionizable |
Analyzing Compounds | Sulfosuccinic Acid |
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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