HPLC Analysis of Sulfosuccinic Acid on a Primesep S Column

Application Notes: Sulfosuccinic acid was retained on Primesep S HILIC/anion exclusion Primesep S column. Retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Since the compound has no UV activity ELSD was used for monitoring and quantitation.
Application Columns: Primesep S
Application compounds: Sulfosuccinic Acid
Detection technique: UV, LC/MS, ELSD/CAD

 

Condition

Column Primesep S, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Sulfosuccinic Acid

 

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep S

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Sulfosuccinic acid

Application Detection:
UV Detection
ELSD/MS Detection