HPLC Analysis of Sulfosuccinic Acid on a Primesep S Column

Application Notes: Sulfosuccinic acid was retained on Primesep S HILIC/anion exclusion Primesep S column. Retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Since the compound has no UV activity ELSD was used for monitoring and quantitation.
Application Columns: Primesep S
Application compounds: Sulfosuccinic Acid
Detection technique: UV, LC/MS, ELSD/CAD

 

Condition
Column Primesep S, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
 Acid, Hydrophilic, Ionizable
Analyzing Compounds Sulfosuccinic Acid

 

Application Column

Primesep S

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Sulfosuccinic acid
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.