| CAS Number | 124832-26-4 |
|---|---|
| Molecular Formula | C13H20N6O4 |
| Molecular Weight | 324.342 |
| InChI Key | HDOVUKNUBWVHOX-QMMMGPOBSA-N |
| LogP | -0.521 |
| Synonyms |
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Applications:
HPLC Separation of Antiviral Drugs on Primesep 100 Column
April 1, 2021
Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir, Oseltamivir, Valacyclovir
Unlike antibiotic drugs, which actively destroy their targets, antiviral drugs generally prevent the reproduction of their viral targets. Acyclovir, Valacyclovir, and Oseltamivir are 3 antiviral drugs commonly prescribed to treat viral infections. Acyclovir and Valacyclovir are used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. They can also be used to treat chickenpox in children. Oseltamivir is generally used to help treat (or if taken early enough, prevent) influenza (the flu) infections.
Acyclovir, Valacyclovir, and Oseltamivir can be retained on a Primesep 100 mixed-mode column with great peak shape using acetonitrile (ACN), water and a gradient of sulphuric acid (H2SO4) as a buffer. This method can be UV detected at 200 nm with very high resolution.
| Column | Primesep 100, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | Gradient H2SO4 – 0.05- 0.8%, 10 min |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 255 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Acyclovir, Valacyclovir, Oseltamivir |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Valacyclovir
HPLC Determination of Valacyclovir on Primesep 100 Column
March 31, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Valacyclovir
Valacyclovir belongs to a class of drugs called antiviral drugs. Valacyclovir is used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes as well as shingles. It can also be used to treat chickenpox in children. Valacyclovir can be retained on a Primesep 100 mixed-mode column with great peak shape using an isocratic method of acetonitrile (ACN), water and sulphuric acid (H2SO4) as a buffer. UV Detection 255nm.
| Column | Primesep 100, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 255 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Valaciclovir |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Separation of Valacyclovir on Newcrom R1 HPLC column
February 16, 2018
Valacyclovir can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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