| CAS Number | 538-23-8 |
|---|---|
| Molecular Formula | C27H50O6 |
| Molecular Weight | 470.692 |
| InChI Key | VLPFTAMPNXLGLX-UHFFFAOYSA-N |
| LogP | 8.36 |
| Synonyms |
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Applications:
HPLC-MS Method for Analysis of Lipid Standards: Triglyceride Mixtures on Lipak Column
February 25, 2025
HPLC Method for Tricaprylin, Tricaprin, Trimyristin, Trilaurin, Tripalmitin on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tricaprylin, Tricaprin, Trimyristin, Trilaurin, Tripalmitin
Series of triglycerides, which are esters derived from glycerol and three fatty acids.
- Tricaprylin:
- Composed of glycerol and three caprylic acid (C8:0) molecules.
- Found in coconut oil and palm kernel oil.
- Used in cosmetics, food, and pharmaceuticals for its quick energy release.
- Tricaprin:
- Made from glycerol and three capric acid (C10:0) molecules.
- Occurs naturally in coconut oil and goat milk.
- Utilized for energy supplementation and in lipid-based formulations.
- Trilaurin:
- Formed by glycerol and three lauric acid (C12:0) molecules.
- Present in coconut and palm kernel oils.
- Known for its antimicrobial properties and use in soaps and cosmetics.
- Trimyristin:
- Consists of glycerol and three myristic acid (C14:0) molecules.
- Major component of nutmeg butter.
- Employed in the production of surfactants and as a fat source in food.
- Tripalmitin:
- Made up of glycerol and three palmitic acid (C16:0) molecules.
- Found in palm oil, cocoa butter, and animal fats.
- Used in food processing, cosmetics, and as a model lipid in research.
Tricaprylin, Tricaprin, Trimyristin, Trilaurin, Tripalmitin can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of methanol (MeOH), ethanol (EtOH), ammonium formate and formic acid as a buffer. Detection is achieved using LC_MS.
| Column | Lipak, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeOH/EtOH – 60/40-10/90% in 10 min |
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 0.5 ml/min |
| Detection | ESI-SIM: [M+NH4]⁺ – (488, 530, 656, 741, 825); [M + HCOOH + H]⁺ – (685, 853) |
| Class of Compounds | Lipid Standards: Triglyceride Mixtures |
| Analyzing Compounds | Tricaprylin, Tricaprin, Trimyristin, Trilaurin, Tripalmitin |
Application Column
Tricaprylin
Trilaurin
Trimyristin
Tripalmitin
Separation of Tricaprylin on Newcrom R1 HPLC column
February 16, 2018
Tricaprylin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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