| CAS Number | 2649467-58-1 |
|---|---|
| Molecular Formula | C21H20F5N3O4 |
| Molecular Weight | 473.4 |
| InChI Key | XSQUJFKRXZMOKA-PAFIKIDNSA-N |
| LogP | 3 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Suzetrigine
February 17, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC-MS Method for Analysis of Suzetrigine on Primesep SB Column
February 13, 2025
HPLC Method for Suzetrigine on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Suzetrigine
Suzetrigine is a selective NaV1.8 sodium channel inhibitor designed for pain management. By targeting peripheral sensory neurons, it reduces pain transmission while minimizing central nervous system effects. Its unique selectivity and novel mechanism make it a valuable compound for pharmaceutical research and development.
Suzetrigine can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate. Detection is performed using UV at 250 nm, ELSD or ESI-SIM: [M+H]⁺ 474.
| Column | Primesep SB, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV at 250 nm; ESI-SIM: [M+H]⁺ 474 |
| LOD | 8.5 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Sodium Channel Blocker |
| Analyzing Compounds | Suzetrigine |
Application Column
Primesep SB
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
CAD Detection
UV Detection
LC MS Detection
HPLC Method for Analysis of Suzetrigine on Primesep B Column
February 12, 2025
HPLC Method for Suzetrigine on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Suzetrigine
Suzetrigine is a selective NaV1.8 sodium channel inhibitor designed for pain management. By targeting peripheral sensory neurons, it reduces pain transmission while minimizing central nervous system effects. Its unique selectivity and novel mechanism make it a valuable compound for pharmaceutical research and development.
Suzetrigine can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 250 nm.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| LOD | 7.1 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Sodium Channel Blocker |
| Analyzing Compounds | Suzetrigine |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
