HPLC Method for Suzetrigine on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Suzetrigine
Suzetrigine is a selective NaV1.8 sodium channel inhibitor designed for pain management. By targeting peripheral sensory neurons, it reduces pain transmission while minimizing central nervous system effects. Its unique selectivity and novel mechanism make it a valuable compound for pharmaceutical research and development.
Suzetrigine can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate. Detection is performed using UV at 250 nm, ELSD or ESI-SIM: [M+H]⁺ 474.
| Column | Primesep SB, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV at 250 nm; ESI-SIM: [M+H]⁺ 474 |
| LOD | 8.5 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Sodium Channel Blocker |
| Analyzing Compounds | Suzetrigine |
Application Column
Primesep SB
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
CAD Detection
UV Detection
LC MS Detection
