Sucrose Hexasulfate

Molecular FormulaC12H16K6O29S6
Molecular Weight1051.2
InChI KeyVIMPGLZYQCCLIC-NVBZBKOESA-H
Synonyms
  • 386229-70-5
  • Sucrose Hexasulfate hexapotassium salt

Applications:

HPLC Method for Analysis of  Sucrose hexasulfate on BIST A+  Column

November 27, 2023

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Sucrose octasulfate on BIST A+ Column by SIELC Technologies

HPLC Method for Analysis of Sucrose octasulfate on BIST A+ Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Sucrose hexasulfate

Sucrose hexasulfate is a chemically modified form of sucrose, where six of the hydroxyl groups in the sucrose molecule are replaced with sulfate groups. This modification significantly alters the properties of the original sugar molecule.

  1. Structure: Sucrose is a disaccharide made up of glucose and fructose units. In sucrose hexasulfate, six of the hydroxyl (–OH) groups present in these sugar units are substituted with sulfate (–SO4) groups. This sulfation changes the molecule’s chemical behavior.
  2. Properties: The introduction of sulfate groups makes sucrose hexasulfate more acidic and highly polar compared to regular sucrose. These properties can affect its solubility, reactivity, and interactions with other molecules.
  3. Uses and Applications: Sucrose hexasulfate, like other sulfated polysaccharides, may have specialized applications in various fields, including biomedical research, pharmaceuticals, and potentially in industrial processes. These applications exploit the unique properties imparted by the sulfate groups.
  4. Biological and Environmental Impact: Sulfated derivatives of carbohydrates, including sucrose hexasulfate, can have different biological activities compared to their non-sulfated counterparts. They might interact differently with biological systems and could have distinct environmental impacts.
  5. Synthesis and Availability: The synthesis of sucrose hexasulfate is more complex than that of simple sugars, and it is typically produced for specific research or industrial purposes rather than being widely available as a commodity chemical.

Sucrose hexasulfate is a modified form of sucrose with unique chemical and physical properties due to the presence of sulfate groups, and its applications are more specialized compared to regular sucrose.

Using SIELC’s newly introduced BIST™ method, sucrose hexasulfate can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as DMP acetate, which acts as a bridge, linking the negatively charged dye to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, oligonucleotide can be separated, retained, and detected at ELSD

Please read more on oligonucleotides analysis by HPLC in our April’s 2023 newsletter.

Condition

ColumnBIST A+, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseGradienr MeCN – 70-40%, 15 min
BufferDMP acetate pH 4.0 – 5 mM
Flow Rate0.4 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 70°C,
with a gas flow rate of 1.6 Standard Liters per Minute (SLM
Sample1 mg/ml in MeCN/H2O – 50/50%
Injection volume5 µl
LOD*
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsSulfated polysaccharides
Analyzing CompoundsSucrose Hexasulfate

Application Column

BIST A+

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Sucrose Hexasulfate

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.