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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Analysis of Metamizole, Methylaminoantipyrine on Primesep SB by SIELC Technologies

Metamizole, also known as Analgin or dipyrone, is a non-opioid analgesic and antipyretic widely used for the treatment of pain, fever, and spasms. It belongs to the pyrazolone class of drugs and is valued for its strong analgesic and spasmolytic properties. Unlike NSAIDs, Metamizole has minimal anti-inflammatory effects, making it distinct in its mechanism of action. However, its use is restricted or banned in some countries due to the risk of agranulocytosis, a rare but serious blood disorder.

After administration, Metamizole undergoes rapid hydrolysis in the body, forming its active metabolite 4-Methylaminoantipyrine (4-MAA). This metabolite is primarily responsible for the drug’s analgesic and antipyretic effects. 4-MAA is further metabolized into other derivatives, such as 4-Formylaminoantipyrine (4-FAA) and 4-Acetylaminoantipyrine (4-AAA), which contribute to the overall pharmacological activity of Metamizole.

Despite safety concerns, Metamizole (Analgin) remains widely used in several countries, particularly in Europe, Latin America, and parts of Asia, due to its effectiveness in managing moderate to severe pain. Ongoing research continues to evaluate its safety profile, mechanisms of action, and potential therapeutic applications.

Metamizole, Methylaminoantipyrine can be retained, and analyzed using a Primesep B mix mode stationary phase column. The analysis utilizes a isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is carried out using UV.