| CAS Number | 34123-59-6 |
|---|---|
| Molecular Formula | C12H18N2O |
| Molecular Weight | 206.290 |
| InChI Key | PUIYMUZLKQOUOZ-UHFFFAOYSA-N |
| LogP | 2.87 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Isoproturon on Primesep 100 Column
August 7, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Isoproturon on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Isoproturon
Isoproturon is classified as a phenylurea herbicide. It is used primarily for controlling annual grasses and broadleaf weeds in cereal crops such as wheat and barley. Isoproturon works by inhibiting photosynthesis in the targeted plants, which eventually leads to their death. It is absorbed by the roots and shoots of the plants and is most effective when applied before or during the early stages of weed growth.
Isoproturon can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Isoproturon and information about its various lambda maxima by visiting the following link.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 45% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Samples | 1.0 mg/ml in MeCN – 100% |
| Injection volume | 1 µl |
| LOD* | 10 ppb (250 nm) |
| Class of Compounds | phenylurea herbicide |
| Analyzing Compounds | Isoproturon |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Isoproturon on Newcrom R1 HPLC column
February 16, 2018
Isoproturon can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
HPLC Method for Analysis of Pesticides: Isoproturon, Diuron, Acenaphthene, Carbendazim
July 11, 2017
Isoproturon is an agricultural herbicide with a tendency to adsorb to soils, which leads to it entering bodies of water. In that water, it is highly toxic for algae and oysters, while long term exposure to the pesticide affects the growth of fish. Diuron is a pre-emergent that is used for non-crop areas as well as with many agricultural crops. It has a low acute toxicity to mammals. It can cause irritation of nose and throat.if inhaled and irritation of eyes if comes in contact with the eyes. Acenaphthene is derived from coal tar and has many used such as dyes, pharmaceuticals, insecticides, fungicides, and plastics. It is harmful if ingested, inhaled or comes in contact with skin, and can cause irritations to skin, eyes, upper respiratory tract, and mucous membranes Carbendazim, also known as mercazole, is a broad-spectrum benzimidazole fungicide. It’s used mostly as a worm control agent in places like tennis courts and golf greens. Sometimes it is also used to control plant diseases in cereals and fruits. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Isoproturon, Diuron, Acenaphthene, and Carbendazim. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Primesep 100, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 35-85%, 6 min , 4 min hold |
| Buffer | Gradient H2SO4 – 0.05- 0.15%, 6 min, 4 min hold |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Herbicide, Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Isoproturon, Diuron, Acenaphthene, Carbendazim |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCarbendazim
Diuron
Isoproturon
