HPLC Separation Of Mixture Of Pyridylacetic Acids (2-Pyridylacetic acid, 3-Pyridylacetic acid, 4-Pyridylacetic acid) On Primesep N Column

Condition

Column Primesep N, 4.6×150 mm, 5 µm, 100A

Mobile Phase MeCN/H2O – 80/20%

Buffer AmFm pH 4.0- 20 mM

Flow Rate 1.0 ml/min

Detection UV, 270 nm

Description

Class of Compounds
Acid, Hydrophilic, Ionizable, Metabolite of nicotine and other tobacco alkaloids

Analyzing Compounds 2-Pyridylacetic acid hydrochloride
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride

Condition
Column	Primesep N, 4.6×150 mm, 5 µm, 100A
Mobile Phase	MeCN/H2O – 80/20%
Buffer	AmFm pH 4.0- 20 mM
Flow Rate	1.0 ml/min
Detection	UV, 270 nm

Description
Class of Compounds	Acid, Hydrophilic, Ionizable, Metabolite of nicotine and other tobacco alkaloids
Analyzing Compounds	2-Pyridylacetic acid hydrochloride 3-Pyridylacetic acid hydrochloride 4-Pyridylacetic acid hydrochloride

Application Column

Primesep N

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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2-Pyridylacetic acid hydrochloride
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride

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HPLC Separation Of Mixture Of Pyridylacetic Acids (2-Pyridylacetic acid, 3-Pyridylacetic acid, 4-Pyridylacetic acid) On Primesep N Column

Condition

Description

Application Column

Primesep N

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