HPLC Separation of Mixture of Carbocisteine, Methylparaben & Propylparaben on Primesep 100 Column

Carbocysteine is a medication that reduces the viscosity of mucus making it easier to cough up. Methylparaben and propylparaben are used as preservatives in cosmetics, pharmaceuticals and food. They can be separated in HPLC on a Primesep 100 mixed-mode column by both hydrophobic and ionic properties present on the stationary phase. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN  - 2-50%, 20 min
Buffer H2SO4 - 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm


Class of Compounds Drug,  Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic, Benzoate, Hydroxy Acid, Amino Acid, Hydroxybenzoate, Phenol
Analyzing Compounds Carbocisteine, Methylparaben, Propylparaben
Application Analytes:
Methylparaben sodium
Propylparaben sodium