HPLC Method for Separation PEI and PVP on PVP Column

HPLC Method for separation PVP, Polyethylenimine PEI on PVP by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of PVP, Polyethylenimine PEI

PVP

Polidone Iodide, more commonly known as Povidone-Iodine (PVP-I), is a widely used antiseptic and disinfectant. It is a complex of iodine with polyvinylpyrrolidone (PVP), designed to provide sustained release of iodine for antimicrobial action.

☑️ Polyvinylpyrrolidone (PVP):

Polyvinylpyrrolidone (PVP), a multifunctional synthetic polymer, finds broad utility in life science and biochemical research. This water-soluble polymer serves various roles:

  • Stabilizing agent
  • Emulsifier
  • Suspending agent
  • Solubilizing agent
  • Protective colloid
  • Complexing agent

Its protective colloid properties are particularly valuable in stabilizing proteins, enzymes, and other biological substances, making it ideal for forming stable complexes with iodine in medical formulations

PVP, Polyethylenimine PEI can be retained and analyzed using the PVP column. The analysis utilizes an step gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate. Detection is performed using ELSD.

ColumnPVP, 2.1 x 50 mm, 5 µm, 100 A, dual ended
Mobile PhaseStep gradient MeCN/H2O
BufferAmmonium Acetate  pH 4.0 –  20 mM
Flow Rate0.2 ml/min
DetectionELSD
LODPVP 0.5 ppm, PEI 1.7 ppm

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsPolymer
Analyzing CompoundsPVP, Polyethylenimine PEI

Application Column

PVP

Column Diameter: 2.1 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
PVP
Polyethylenimine PEI
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.