HPLC Method for Analysis of  Sucrose octasulfate on BIST A+  Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Sucrose octasulfate on BIST A+ Column by SIELC Technologies

HPLC Method for Analysis of Sucrose octasulfate on BIST A+ Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Sucrose octasulfate

Sucrose octasulfate is a chemical compound derived from sucrose, a common sugar. It is characterized by the substitution of eight sulfate groups for hydroxyl groups in the sucrose molecule. This modification significantly changes the properties of the original sugar molecule, making sucrose octasulfate more complex and specialized in its applications.

In terms of its structure, sucrose is a disaccharide composed of glucose and fructose units. When these hydroxyl groups are replaced with sulfate groups in sucrose octasulfate, the molecule becomes highly polar and acidic due to the presence of sulfate groups, which are strong acid residues.

Using SIELC’s newly introduced BIST™ method, sucrose octasulfate can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as TMEDA formate, which acts as a bridge, linking the negatively charged dye to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, oligonucleotide can be separated, retained, and detected at ELSD

Please read more on oligonucleotides analysis by HPLC in our April’s 2023 newsletter.

Condition

ColumnBIST A+, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseGradienr MeCN – 70-40%, 15 min
BufferDMP acetate pH 4.0 – 5 mM
Flow Rate0.4 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 70°C,
with a gas flow rate of 1.6 Standard Liters per Minute (SLM
Sample1 mg/ml in MeCN/H2O – 50/50%
Injection volume5 µl
LOD*
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsSulfated polysaccharides
Analyzing CompoundsSucrose octasulfate

Application Column

BIST A+

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Sucrose octasulfate
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.