Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ellagic Acid
Ellagic acid (EA) is a naturally occurring polyphenol. The best sources of ellagic acid in the diet are pomegranate, strawberries, raspberries, blackberries, and walnuts. It has been investigated extensively because of its antiproliferative action in some cancers, along with its anti-inflammatory effects. Ellagic acid can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of methanol (MeOH) and water with perchloric acid (HClO4) buffer. The analysis method can be UV detected at 253 nm.
| Column | Newcrom BH, 2.1×100 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 40/60% |
| Buffer | HClO4 – 0.5% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 253 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Ellagic Acid |
Application Column
Newcrom BH
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
Ellagic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
