HPLC Determination of TFA on Newcrom BH Column

HPLC Method for TFA (Trifluoroacetic Acid) on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of TFA (Trifluoroacetic Acid).

Trifluoroacetic Acid (TFA) is a synthetic organofluorine acid with the chemical formula C2HF3O2. It is a substitution derivative of acetic acid, with fluorine atoms replacing the hydrogen atoms of the acetyl group. It is also a much stronger acid than acetic acid. TFA is widely used in organic synthesis as well as ion pairing reagent or buffer in HPLC. It is corrosive and toxic to aquatic life and mammals, causing severe irritation and burns to skin, eyes, and the respiratory tract. Not only that, TFA is also highly mobile and persistent, leading to high retention of it in soil and water. Determination of it’s threat level on the environmental and health levels are still ongoing.

TFA can be detected at low UV. Using Newcrom BH mixed-mode column, a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer can be used to retain TFA and UV detect it at 200nm.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description
Class of Compounds Acid
Analyzing Compounds TFA (Trifluoroacetic Acid)

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
TFA (Trifluoroacetic Acid)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.