High Performance Liquid Chromatography (HPLC) Method for Analysis of Buprenorphine and Naloxone on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Buprenorphine is an opioid partial agonist used to treat Opioid Use Disorder (OUD). Naloxone is used to treat the effects of opioids as part of a treatment for opiate addictions. When placed together in a sublingual film, they can be quite effective at treating opiate/narcotic addictions non-addictively. Buprenorphine and Naloxone can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 280 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Buprenorphine and Naloxone
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV,280 nm |
| Peak Retention Time | 3.8, 8.2 min |
Description
| Class of Compounds | Drugs |
| Analyzing Compounds | Buprenorphine, Naloxone |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
BuprenorphineBuprenorphine hydrochloride
Naloxone
Naloxone chlorhydrate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
