HPLC Method for Analysis of Hydrocortisone

Chr_1055




Hydrocortisone, also known as 11β,17α,21-trihydroxypregn-4-ene-3,20-dione, is a corticosteroid, is similar to a natural hormone produced by your adrenal glands. It is used to treat certain forms of arthritis as well various skin conditions (redness, swelling, and itching). Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Hydrocortisone. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
HPLC Ananlysis of Hydrocortisone
HPLC Method for Analysis of Hydrocortisone
HPLC Separation of Hydrocortisone and Hydrocortisone’s Degradation Products
Hydrocortisone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.