o-Toluidine

o-Toluidine structural formula

CAS Number95-53-4
Molecular FormulaC7H9N
Molecular Weight107.156
InChI KeyRNVCVTLRINQCPJ-UHFFFAOYSA-N
LogP1.32
Synonyms
  • 2-Methylaniline
  • Benzenamine, 2-methyl-
  • 95-53-4
  • 2-Methylbenzenamine
  • o-Toluidine
  • Benzenamine, 2-methyl-
  • 1-Amino-2-methylbenzene
  • 2-Amino-1-methylbenzene
  • 2-Aminotoluene
  • 2-Methyl-1-aminobenzene
  • 2-Methylphenylamine
  • 2-Tolylamine
  • ANILINE, 2-METHYL-
  • NSC 15348
  • o-Aminotoluene
  • o-Methylaniline
  • o-Methylbenzenamine
  • ORTHO-TOLUIDINE
  • o-Toluidin
  • o-toluidina
  • o-Tolylamine
  • TOLUIDINE, O-
  • Toluidine, ortho-
  • EINECS 202-429-0
  • RCRA waste number U328
  • o-Toluidyna
  • UNII-B635MZ0ZLU
  • 1-Methyl-2-aminobenzene
  • 2-Methylbenzamine
  • 2-Toluidine

Applications:

Separation of Toluidine and Aminobiphenyl Isomers

July 6, 2015

Toluidine isomers and aminobyphenyl isomers are organic compounds each with a substituted amine group. Primesep 200 was used to separate these isomers. Primesep 200 separates isomers by interacting with polar molecules, retaining basic compounds, and additionally by reverse phase mechanism.

Condition

Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm Ph 3.5
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Hydrophobic, Ionizable
Analyzing Compounds o-Toluidine, 2-Aminobiphenyl, m-Toluidine, p-Toluidine, 4-Aminobiphenyl

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Aminobiphenyl
4-Aminobiphenyl
m-Toluidine
o-Toluidine
p-Toluidine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Toluidine Isomers in Cation-Exchange Mode

July 14, 2011

chr_307.gif

Isomers of toluidine were successfully retained and separated in cation-exchange mode on a Primesep S2 column. Primesep S2 column is a HILIC/cation-exchange column that can be operated in HILIC, cation-exchange, and anion-exclusion modes. Method and column are compatible with ELSD/LC/MS and UV and can be used for routine analysis of polar molecules.

Application Column

Primesep S2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Toluidines
m-Toluidine
o-Toluidine
p-Toluidine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Toluidine Isomers on Primesep 200 Coulmn

December 31, 2005

Toluidine isomers are based on toluene and differ whether the primary amine is ortho, meta or para to the methyl group. A Primesep 200 column with an ammonium formate buffer or a Primesep 100 column with sodium phosphate baseline resolves the three regioisomers. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate, or sodium phosphate and ultraviolet (UV) detection at 250 nm.

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Toluidines
m-Toluidine
o-Toluidine
p-Toluidine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Toluidine Isomers on Primesep 100 Column

June 12, 2004

Toluidine isomers are based on toluene and differ whether the primary amine is ortho, meta or para to the methyl group. A Primesep 200 column with an ammonium formate buffer or a Primesep 100 column with sodium phosphate baseline resolves the three regioisomers. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate, or sodium phosphate and ultraviolet (UV) detection at 250 nm.

Application Analytes:
m-Toluidine
o-Toluidine
p-Toluidine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.