2,4-D Retention on Primesep 100 and Obelisc R

  2,4-D makes up half of the formula for Agent Orange, the defoliant that was used during the Vietnam War and Malayan Emergency. Commonly used in lawn and turf pesticides, 2,4-D is typically mixed with other pesticides such as mecoprop and dicamba. It was analyzed on Primesep 100 and Obelisc R. Primesep 100 separates using reverse-phase and acidic ion-pairing groups. Obelisc R has many modes of separation which allow it to tune retention. Method is LC/MS compatible and conditions could be replicated to retain many pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%
Buffer Gradient AmAc pH 3.0- 30-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Pesticide, Hydrophobic, Ionizable
Analyzing Compounds 2,4-D
 
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
2,4-Dichlorophenoxyacetic acid

Application Detection:
UV Detection