If you are looking for optimized HPLC method to analyze 3-Pyridylacetic acid hydrochloride check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide 

Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.

Nicotinic acid, also known as niacin and vitamin B3, is a water-soluble B vitamin with C₆H₅NO₂ molecular formula. It is crucial for energy production.

Derrivatives are compounds formed when the parent compound goes through a chemical recation, replacing atoms with different ones, leading to different physical and chemical properties.

2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.

HPLC Method for 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride on Primesep C by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride

Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.

2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride can be retained and analyzed using the Primesep C stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

Column	Primesep C, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase	MeCN/H2O – 10/90%
Buffer	AmFm pH 4.0- 10 mM
Flow Rate	1.0 ml/min
Detection	UV, 270 nm

Class of Compounds	Acid, Hydrophilic, Ionizable,  Metabolite of nicotine and other tobacco alkaloids
Analyzing Compounds	2-Pyridylacetic acid hydrochloride 3-Pyridylacetic acid hydrochloride 4-Pyridylacetic acid hydrochloride

HPLC Method for 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride on Primesep N by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride 

Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.

2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride can be retained and analyzed using the Primesep N stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

Column	Primesep N, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase	MeCN/H2O – 80/20%
Buffer	AmFm pH 4.0- 20 mM
Flow Rate	1.0 ml/min
Detection	UV, 270 nm

Class of Compounds	Acid, Hydrophilic, Ionizable,  Metabolite of nicotine and other tobacco alkaloids
Analyzing Compounds	2-Pyridylacetic acid hydrochloride 3-Pyridylacetic acid hydrochloride 4-Pyridylacetic acid hydrochloride