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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

HPLC Method for 2,3-Dichlorobenzoic anhydride on Primesep S2 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2,3-Dichlorobenzoic anhydride.

2,3-Dichlorobenzoic Anhydride is an impurity with the chemical formula C₁₄H₆Cl₄O₃. It is formed during the production of Lamotrigine, anticonvulsant used to treat epileptic seizures and Bipolar Disorder. Removing 2,3-Dichlorobenzoic Anhydride is paramount for maximizing Lamotrigine purity. It had no known uses outside of quality control. You can find detailed UV spectra of 2,3-Dichlorobenzoic anhydride and information about its various lambda maxima by visiting the following link.

2,3-Dichlorobenzoic anhydride can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.