| CAS Number | 944-73-0 |
|---|---|
| Molecular Formula | C7H8N4O3 |
| Molecular Weight | 196.164 |
| InChI Key | OTSBKHHWSQYEHK-UHFFFAOYSA-N |
| LogP | -0.520 |
| Synonyms |
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Applications:
HPLC Method for Analysis mixture of Xanthinesand Uric Acid BIST B+ by SIELC Technologies
November 16, 2022
HPLC Method for Analysis mixture of Xanthines and Uric Acid BIST B+ by SIELC Technologies.
Separation type: Hydrophilic interaction liquid chromatography (HILIC)
Xanthines and uric acid are related compounds in the body and both are involved in the metabolism of purines.
Xanthines are a group of alkaloids that are widely distributed in plants, and also occur in the tissues and fluids of animals. They are known to stimulate the central nervous system and cardiac muscle, and also have diuretic effects. Some commonly known xanthines include caffeine (found in coffee, tea, and chocolate), theobromine (found in cocoa and chocolate), and theophylline (used as a drug in the treatment of respiratory diseases like asthma).
In the body, xanthines are intermediates in the degradation of adenosine monophosphate to uric acid. This metabolic pathway starts with adenosine monophosphate (AMP), which is deaminated to form inosine monophosphate (IMP). IMP is then converted into a xanthine known as hypoxanthine. Hypoxanthine is then oxidized to xanthine, and finally, xanthine is further oxidized to uric acid. Both of the oxidation steps are catalyzed by the enzyme xanthine oxidase.
Uric acid and Xanthines can be retained, analyzed, and separated using an isocratic analytical method on a BIST B+ column. The simple mobile phase for this method comprises water, acetonitrile (MeCN), and formic acid as an ionic modifier. The analytical method can be monitored with UV detection at 260 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)
Condition
| Column | BIST B+, 4.6 x 150 mm, 10 µm, 100 A |
| Mobile Phase | MeCN – 85% |
| Buffer | FA – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260, 290 nm |
| Peak Retention Time | 2.01, 3.02, 4.2, 9.09 min |
Description
| Class of Compounds | Acid, Xanthines |
| Analyzing Compounds | Uric Acid, Caffeine, 1,3-Dimethyluric acid, Xanthine, Hypoxanthine |
Application Column
BIST B+
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 10 µm
Pore Size: 100 A
Caffeine
Hypoxanthine
Uric acid
Xanthine
Separation of 1,3-Dimethyluric acid on Newcrom R1 HPLC column
May 16, 2018
1,3-Dimethyluric acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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