Tetramethylammonium

HPLC Method for Analysis of Tetramethylammonium on (Column not found) by SIELC Technologies

Tetramethylammonium is a quaternary ammonium cation with the formula (CH3)4N+. This compound is one of the simplest quaternary ammonium ions, and is notable for its prevalence in both science and industry.

The central nitrogen atom in tetramethylammonium has a positive charge due to the four alkyl (methyl, CH3) groups attached to it. This makes it a cation, meaning it carries a positive charge.

This compound is often used in its chloride salt form (tetramethylammonium chloride), which is a white crystalline powder that is highly soluble in water and used in various chemical applications, such as phase transfer catalysts, surfactants, and reagents in organic synthesis.

Tetramethylammonium ions can also serve as counter ions or blocking ions in certain biological and chemical studies. For example, they are sometimes used to block potassium channels in studies of cellular physiology.

The tetramethylammonium can be retained, analyzed, on a Newcrom AH column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium formate (AmFm) ionic modifier. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Tetramethylammonium

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Description

HPLC Method for Analysis of Tetramethylammonium, Sodium, Potassium on Newcrom AH by SIELC Technologies

Tetramethylammonium chloride is a quaternary ammonium salt used widely as a reagent in industrial applications.  It can be separated from sodium and potassium chlorides on a mixed-mode Newcrom AH column with a simple isocratic MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.

Hydrophilic and hydrophobic quaternary amines, along with sodium ion, were separated by mixed-mode chromatography on a Primesep 200 column. Mechanism of retention for sodium and tetramethylammonium ions is cation exchange, while the tetrabutylammonium ion is retained by combination of reversed-phase and cation-exchange mechanisms. All three compounds are not UV-active and monitoring is done by ELSD/CAD.

Primesep C separates a mixture of quaternary amines by a combination of cation exchange, complex formation, and hydrophic interactions. Methyltriethylammonium, tetraethylammonium, and tetramethylammonium cations as well as bromide counter ion are separated on a short 50 mm column. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and triethylamine acetate with evaporative light scattering detection (ELSD).